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瓦萨比 (Eutrema japonicum) 基因组的染色体水平组合,通过哈普洛型解析

Hiroyuki Tanaka¹, Tatsuki Hori¹, Shohei Yamamoto²

¹School of Life Science and Technology, Tokyo Institute of Technology, Meguro-ku, Tokyo, 152-8550, Japan.

Scientific data

|July 11, 2023

概括

研究人员已经对传统日本调味料瓦萨比 (Eutrema japonicum) 的基因组进行了测序. 这种高质量的参考基因组为了解瓦萨比提供了宝贵的资源.

科学领域:

基因组学就是基因组学.
植物科学植物科学
植物学植物学

背景情况:

瓦萨比 (Eutrema japonicum) 是一种特有物种,也是日本重要的传统调味品.
了解瓦萨比的遗传构成对于其保护和繁殖至关重要.

研究的目的:

为了生成一个高质量的,染色体水平,和哈普洛型解析的参考基因组Eutrema japonicum.
提供一个有价值的遗传资源,用于研究化学生态,进化和 Wasabi 的繁殖.

主要方法:

使用了PacBio CLR (连续长读),Illumina和Hi-C测序技术.
进行了读取映射和族系分析,用于亚基因组和单种类型的分配.
使用基准测试验证了基因组质量和完整性,使用了通用单拷贝正统学,Merqury和Inspector.

主要成果:

成功组装了E. japonicum的染色体级基因组,包括28个染色体和1512.1Mb的序列数据.
实现了高架N50长度为55.67Mb,表明一个连续的基因组组装.
通过严格的验证,与之前发布的基因组组合相比,证明了更高的质量和完整性.

结论:

生成的参考基因组代表了E. japonicum基因组学的重大进步.
这一资源将促进未来关于Eutrema和Brassicaceae的化学生态和演变的研究.
预计基因组组装将加速改进的瓦萨比品种的育种计划.

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