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转录基因分析揭示了Fragaria nilgerrensis Schlecht中安托素变化的基础机制. 和其跨物种混合物

Aihua Wang^1,2, Hongye Ma², Xingtao Zhang¹

¹School of Biological and Food Engineering, Engineering Research Center for Development and High Value Utilization of Genuine Medicinal Materials in North Anhui Province, Suzhou University, Suzhou, 234000, Anhui, China.

BMC plant biology

|July 11, 2023

概括

弗拉加利亚·尼尔格伦西斯 (Fragaria nilgerrensis) 的跨物种杂交物种由于佩拉戈尼丁衍生物而积累了淡红色的水果颜色. 像DFR和BZ1这样的关键基因,以及转录因子,调节这种安东基亚尼丁生物合成,以改善草色彩.

科学领域:

植物遗传学植物遗传学
生物化学生化学
分子生物学分子生物学

背景情况:

草 (Fragaria nilgerrensis,简称FN) 是草育种的宝贵遗传资源.
水果的颜色是影响消费者偏好的关键特征.
在FN及其杂交品中,水果颜色的遗传基础尚未得到充分研究.

研究的目的:

为了研究基因和生化机制背后的果实颜色在FN跨种杂交的混合物.
为了确定关键的颜料和调节基因,涉及到安托西亚尼丁生物合成.

主要方法:

对FN (白色) 和其混合物 (淡红色) 的比较转录组分析.
黄胺含量分析.
基因表达和色素积累之间的相关性分析.

主要成果:

确定了pelargonidin-3-O-glucoside和pelargonidin-3-O-rutinoside作为浅红色水果中的潜在关键色素.
观察到二黄4-减少酶 (DFR) 和安东基亚尼丁3-O-葡萄糖转移酶 (BZ1) 基因的显著上调.
在转录因子家族 (MYB,bHLH,WRKY等) 中检测到差异性表达. 和与DFR和BZ1.1的相关性.

结论:

佩拉戈尼丁衍生物可能是导致淡红色果皮颜色的原因.
DFR,BZ1和特定的转录因子在增强pelargonidin积累方面发挥着至关重要的作用.
研究结果提供了关于草中安东基亚尼丁生物合成调节的见解,以及对水果颜色的遗传改进的潜力.

关键词:

安托西亚宁生物合成弗拉加利亚·尼尔杰伦尼斯斯·施莱赫特跨物种混合物跨物种混合物佩拉戈尼丁的衍生品佩拉戈尼丁的衍生品红色的颜色是红色的

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