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在Ciona胚胎发生过程中,水平转移的甘氨基酸酶基因GH6-1的表达和可能的功能

Kun-Lung Li^1,2, Keisuke Nakashima³, Kanako Hisata³

¹Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa, 904-0495, Japan. kunlung.li@gmail.com.

|July 11, 2023

概括

衣GH6-1是一种水平转移的基因,在胚胎表皮细胞中表达,在纤维素代谢中起着至关重要的作用,影响着衣的发育和形态.

科学领域:

进化生物学进化生物学
基因组学就是基因组学.
生物化学生物化学

背景情况:

衣动物在合成纤维素来制造它们的外衣方面是独一无二的.
锡奥纳肠道菌的基因组包含一个水平获得的纤维素合成酶基因 (CesA).
一个相关的基因,GH6-1,具有潜在的功能性甘氨基酸酶域,被调查其在胚胎发生中的作用.

研究的目的:

为了确定GH6-1是否在Ciona胚胎发生过程中表达.
为了确定GH6-1表达的组织.
调查GH6-1在状细胞发育和纤维素代谢中的作用.

主要方法:

定量逆转录PCR (qRT-PCR) 和现场杂交以分析GH6-1表达模式.
单细胞RNA测序以识别表达GH6-1.
在TALEN中介的基因组编辑中创建GH6-1淘汰赛Ciona幼虫.

主要成果:

GH6-1在尾茎胚胎和幼虫的表皮中表达,反映了CesA的表达.
GH6-1淘汰幼虫表现出异常的粘性乳头,改变了纤维素的分布,并失败了变形.
在表皮细胞中检测到GH6-1表达,有些表达CesA.

结论:

来自水平基因转移的Tunicate GH6-1在胚胎表皮细胞中表达和功能.
切萨和GH6-1都涉及基纤维素代谢,影响形态学和生态学.
GH6-1在虫基因组中的招募突显了它在形动物进化中的作用.

关键词:

在Ciona胚胎生成中的表达和功能.甘氨基酸酶家族-6 GH6基因横向转移的基因是水平转移的突尼西亚国家 (Tunicates)

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