对于潜在的临床应用,肝细胞癌中与铁亡相关的ceRNA网络
Zelong Yang1, Kun He1, Weigang Chen1
1Department of Hepatobiliary Surgery, The First Affiliated Hospital, Airforce Military Medical University Xi'an 710032, Shaanxi, China.
American journal of translational research
|July 12, 2023
概括
这项研究揭示了一种与肝细胞癌 (HCC) 化相关的竞争性内源性RNA (ceRNA) 网络. DNAJC27-AS1和PPIF通过海绵化miR-23b-3p调节铁亡,为HCC治疗提供了潜在的临床应用.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 肝细胞癌 (HCC) 是一个主要的全球健康问题.
- 铁亡是一种受调节的细胞死亡形式,在癌症进展中起着关键作用.
- 了解控制HCC中ferroptosis的调节网络对于开发新疗法至关重要.
研究的目的:
- 调查与肝细胞癌 (HCC) 中铁化相关的竞争性内源性RNA (ceRNA) 网络.
- 探索这种与铁亡相关的ceRNA网络在HCC.中的潜在临床应用.
主要方法:
- 对HCC患者的癌症基因组图谱 (TCGA) 的RNA测序数据进行了分析.
- 单样样本基因组丰富分析 (ssGSEA) 评估了自,热和铁的途径.
- 权重基因共同表达网络分析 (WGCNA) 模块化了lncRNA,miRNA和mRNA,以识别与ferroptosis相关的模块.
- 一个ceRNA网络被构建,一个特定的ceRNA轴 (DNAJC27-AS1/miR-23b-3p/PPIF) 经过实验验证,使用 luciferase 记者测试.
主要成果:
- 在HCC患者中,铁亡水平与整体存活率之间存在显著的相关性.
- 成功构建了一个全面的与铁灭相关的ceRNA网络.
- 实验验证证证实,DNAJC27-AS1和PPIF直接海绵miR-23b-3p,从而降低HCC细胞中的铁灭.
结论:
- 已确定的铁亡相关的ceRNA网络为HCC铁亡的机制提供了有价值的见解.
- 这个网络有望成为促进HCC的理解和临床管理的资源.
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