在储存期间,红细胞的免疫调节特性
Eva Laurén1,2, Lotta Sankkila1, Ville Pettilä2
1Finnish Red Cross Blood Service, Helsinki, Finland.
概括
红细胞 (RBC) 储存介质,而不是细胞外囊泡 (EVs),影响免疫细胞的反应. 从储存的红细胞中获得的超常剂,无论年龄如何,都会改变T细胞的增殖和细胞因子的分泌,这表明它在输血免疫调节中的作用.
科学领域:
- 免疫学 免疫学 免疫学
- 输血医学 输血医学
- 细胞生物学 细胞生物学
背景情况:
- 红细胞 (RBC) 输血可以调节接受者的免疫系统.
- 红细胞储存导致细胞功能受损,细胞外囊泡 (EV) 释放,以及生物活性物质在储存介质中的积累.
- EVs涉及细胞与细胞相互作用,并可能解释输血相关的免疫调节.
研究的目的:
- 为了研究红细胞储存超体 (SN) 和EVs对受体免疫细胞的免疫调节作用.
- 确定红细胞储存年龄是否影响这些免疫调节效应.
主要方法:
- 周围血液单核细胞 (PBMCs) 暴露于全基红细胞SN,EVs,稀释血和储存溶液 (SAGM).
- 使用流式细胞计量评估T细胞激活和增殖.
- 来自LPS刺激的PBMCs的细胞因子分泌量通过ELISA测量.
主要成果:
- 来自新鲜和储存单位的RBC SN诱导了免疫调节,而EV没有.
- 红细胞SN和稀释血增强了CD8+T细胞的增殖和T细胞的激活 (CD69的上调).
- SN抑制单细胞TNF-α和增加IL-10分泌;稀释血增加了两者.
结论:
- 储存的RBC SN具有独立于储存年龄的混合免疫调节效应.
- 新鲜的红细胞,即使只有很少的EV,也可以引起免疫反应.
- 红细胞产品中的残留血可能有助于观察到的免疫调节作用.
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