葡萄糖皮质醇应激反应是否使变得更有毒? 一个关于调节布法迪埃诺利德毒素合成的实验研究
B Üveges1,2, C Kalina1,3, K Szabó4
1Department of Evolutionary Ecology, Plant Protection Institute, Centre for Agricultural Research, Eötvös Loránd Research Network, Herman Ottó út 15, 1022 Budapest, Hungary.
Integrative organismal biology (Oxford, England)
|July 12, 2023
概括
皮质 (CORT) 不调节青bufadienolide毒素的合成. 相反,上游压力激素,而不是CORT本身,可能会控制毒素的产生,以应对环境变化.
科学领域:
- 动物学 动物学
- 化学生态化学生态学
- 内分泌学 在内分泌学.
背景情况:
- 化学防御对于生物体的健康至关重要,但它们的生理调节,特别是脊椎动物中的毒素合成,仍然不太了解.
- 青中防御性毒素布法迪化物可以通过压力因素进行上调,这表明与内分泌应激反应的联系.
- 皮质 (CORT) 是主要的两类葡萄皮质类药物,被假设能刺激布法迪埃诺利德的合成.
研究的目的:
- 调查普通的布法迪埃诺利德合成是否受到皮质 (CORT) 或其上游激活剂的调节.
- 确定CORT和甲基拉 (MTP),一种CORT合成抑制剂在调节不同条件下的布法迪埃诺利德产生的作用.
主要方法:
- 常见的头被外源性CORT (exoCORT) 或MTP治疗了2天或6天,有或没有捕食线索.
- 测量包括CORT释放率和布法迪埃诺利德含量.
- 使用MTP治疗通过负反来刺激CORT的上游调节器.
主要成果:
- 随着exoCORT和在较小程度上,MTP治疗,无论持续时间如何,CORT释放率都增加了.
- 经过6天的exoCORT暴露,bufadienolide含量显著下降.
- 无论是exoCORT (2天) 还是MTP (2或6天) 都没有影响bufadienolide水平;捕食线索没有影响.
结论:
- 升高的皮质类固醇 (CORT) 不会直接刺激普通的布法迪埃诺利德合成.
- 在应对环境挑战时,布法迪埃诺利德合成调节似乎是由压力路径中CORT上游的激素调节的.
- 这些发现挑战了CORT是布法迪埃诺利德生产的主要驱动因素的假设.
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