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黑的可用性诱导了协调的DNA甲基化和基因表达变化在Porphyromonas gingivalis

Ricardo Costeira¹, Joseph Aduse-Opoku², Jon J Vernon³

¹Department of Twin Research and Genetic Epidemiology, King's College London , London, United Kingdom.

|July 12, 2023

概括

牙周病病原体 Porphyromonas gingivalis 显示出改变的 DNA 甲基化和基因表达,以应对 hemin. 这些表观遗传变化调节毒性因素,为牙周炎机制提供了新的见解.

科学领域:

微生物学微生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
口腔健康口腔健康

背景情况:

牙周病是一种慢性炎症状况,与口腔病原体 Porphyromonas gingivalis 有关.
众所周知,P. gingivalis的毒性受到黑的可用性的影响,但调节机制尚不清楚.
细菌DNA甲基化是基因表达的潜在调节者.

研究的目的:

为了描述P. gingivalis的甲基组.
为了研究DNA甲基化和基因表达之间的关系,以应对不同的血红蛋白度.
在牙周病中识别调节P. gingivalis毒性的表观遗传机制.

主要方法:

P. gingivalis W50是在过剩和有限的半球体条件下培养的.
使用纳米孔技术进行全甲基组分析.
使用Illumina RNA-Seq.进行了转录组分析.
对特定动机 (Dam/Dcm) 和一般修改 (6mA,5mC) 的DNA甲基化量化.

主要成果:

过多的血红蛋白 (161个过度表达的,268个表达不足的基因) 观察到基因表达的显著变化.
在对Dam"GATC"基因,N6-甲基氨酸 (6mA) 和5-甲基氨酸 (5mC) 的反应中检测到不同的DNA甲基化特征.
联合分析显示,基因表达和DNA甲基化 (6mA,5mC) 的协调变化影响了参与乳酸利用和ABC转运器的基因.

结论:

黑的可用性显著影响了P. gingivalis.中的DNA甲基化和基因表达.
改变的DNA甲基化模式与半膜调节的毒性因子表达有关.
这些发现阐明了P. gingivalis的新型表观遗传调节机制,有助于理解它在牙周疾病中的作用.

关键词:

通过DNA甲基化.基因表达的基因表达方式铁的可用性铁的可用性口腔生物学口腔生物学牙周病是一种牙周病.毒性毒性是一种毒性.

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