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在老化的大脑小鼠中,Pax6会影响Ras-Raf-ERK1/2

Khushboo Srivastava¹, Rajnikant Mishra²

¹Biochemistry and Molecular Biology Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.

|July 12, 2023

概括

老龄化改变了Pax6,Ras,Raf和ERK1/2在大脑中的表达. Erk1/2 (细胞外信号调节激酶) 与Pax6具有协同活性,影响细胞代谢和生存信号通路.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
衰老研究研究衰老研究

背景情况:

一个关键的转录因子Pax6经历了与年龄相关的变化,并与调节细胞代谢和生存途径的蛋白质相互作用,如Ras-GAP.
在老化过程中,不同大脑区域的Ras,Raf和ERK1/2的特定表达模式在很大程度上仍未被描述.

研究的目的:

研究不同大脑区域的Pax6和各种形式的Ras,Raf和ERK1/2的年龄依赖表达.
探索Pax6与神经质细胞系中的Ras-Raf-ERK1/2通路之间的关联.
分析Pax6对Ras-Raf-ERK1/2表达和活性的影响.

主要方法:

在年轻和老小鼠的大脑区域中对Pax6,Ras,Raf和ERK1/2表达的定量分析.
与神经质细胞系 (PC-12,C6-glia,U-87 MG) 进行共同培养实验,以研究Pax6相互作用.
siRNA介导的Pax6的淘汰,以评估其对Ras-Raf-ERK1/2通路的影响.
通过RT-PCR和 luciferase 记者测定来分析基因表达和蛋白质活性.

主要成果:

在多个大脑区域观察到Pax6,Ras,Raf和ERK1/2表达的显著年龄相关变化.
发现Pax6在共同培养的神经质细胞中与Ras,Raf和ERK1/2相互作用.
通过siRNA介导的Pax6的淘汰影响了Ras,Raf和ERK1/2.2.的表达模式.
ERK1/2与Pax6.6显示出协同作用.

结论:

衰老显著影响Pax6和Ras-Raf-ERK1/2途径组件在大脑中的表达.
Pax6在调节Ras-Raf-ERK1/2信号级联中发挥作用,可能影响神经元功能和生存.
这些发现提供了对大脑衰老和神经保护背后的分子机制的见解.

关键词:

衰老的衰老衰老的衰老在ERK1/2中.潘拉斯 (Pan-Ras) 是一个泛种族.在Pax6中使用Pax6.拉夫拉夫拉夫拉夫拉夫拉夫拉夫拉夫

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