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长期暴露于双A的多重和转录基因变化揭示了雄性斑马鱼的生殖毒性

Xu Luo¹, Zhanxiang Du¹, Jinyuan Hu¹

¹School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, 211816, China.

Fish physiology and biochemistry

|July 12, 2023

概括

双甲 (BPA) 暴露会损害斑马鱼的繁殖,导致精子异常和改变基因表达. 这项研究揭示了BPA.

科学领域:

环境毒理学环境毒理学
水生生物学水生生物学
生殖科学生殖科学

背景情况:

双A (BPA) 是一种广泛存在的污染物,已知对水生有毒.
BPA在水生生物中的跨代生殖效应仍然不清楚.

研究的目的:

研究BPA对斑马鱼丸的形态学,组织学和跨代影响.
确定BPA诱导的生殖毒性背后的分子机制.

主要方法:

斑马鱼被暴露在BPA中.
评估精子参数 (数量,活动,生育能力).
利用RNA测序 (RNA-seq) 进行转录基因分析和基因本体学 (GO) 和通路分析.

主要成果:

暴露于BPA导致精子数量,活动和生育能力下降.
在斑马鱼丸中鉴定了1940个差异表达基因 (DEGs).
对GO的分析显示了与精子相关的功能的丰富 (青素结合, zona pellucida结合,青素反应).
途径分析突出了细胞粘附分子和生物合成途径 (类固醇激素,脂肪酸) 的变化.

结论:

长期暴露于BPA会导致雄性斑马鱼显著的生殖毒性.
多种和转录基因的改变为BPA的生殖损害机制提供了洞察力.
研究结果强调BPA是水生生物的生殖毒素.

关键词:

双 A 是一种双.在RNA-Seqq.精子精子精子测试 (Testis) 是一个测试.斑马鱼是一种斑马鱼.

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