沙林微生物tongyeongense sp. 的描述 从海水中分离出来的nov
Dariimaa Ganbat1, Dong-Woo Lee2, Seong-Bo Kim3
1Major in Food Biotechnology and Research Center for Extremophiles and Marine Microbiology, Silla University, Busan 46958, Republic of Korea.
概括
在海水中发现了一种新的中等性细菌,Salinimicrobium tongyeongense HN-2-9-2T. 这种新型物种扩展了Salinimicrobium属,具有独特的基因组和生化特征.
科学领域:
- 微生物学 微生物学
- 细菌学 细菌学是一门学科.
- 纳税学是一种分类学.
背景情况:
- 从韩国东阳市海水中分离出一种新型细菌菌株,HN-2-9-2T.
- 这种菌株的特征是氏体阴性,非运动性,中等性,杆状细菌.
研究的目的:
- 为了分类新型细菌分离HN-2-9-2T.
- 确定该菌株是否代表Salinimicrobium属内的新物种.
主要方法:
- 从海洋环境中分离和培养细菌.
- 物理化学特征包括生长条件 (NaCl,pH,温度).
- 使用16S rRNA基因测序进行分子遗传学分析.
- 基因组分析包括平均核酸标识 (ANI),平均氨基酸标识 (AAI) 和数字DNA-DNA杂交 (dDDH).
- 对menaquinone,脂肪酸和极性脂质进行化学分类分析.
主要成果:
- 该菌株在0.57%的NaCl,pH值5.58.5和1845°C下表现出最佳的生长.
- 16S rRNA基因序列分析显示,与Salinimicrobium xinjiangense BH206具有最高的相似性 (98.2%).
- ANI (76.0%),AAI (81.9%) 和dDDH (19.7%) 的值证实了它与S. xinjiangense. 的区别.
- 基因组分析显示,基因组大小为3,509,958bp,DNA G+C含量为43.0%.
- 化学分类学分析确定MK-6是唯一的menaquinone和特定的主导脂肪酸和极性脂质.
结论:
- 多相分类学数据强烈支持将HN-2-9-2T分类为新物种.
- 这种新物种的拟议名称是Salinimicrobium tongyeongense sp. 在新的一年里.
- 类型菌株是HN-2-9-2T,加入号为KCTC 82934T和NBRC 115920T.
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