在酵母过氧体中构建和优化非经典异烯酸生物合成途径,用于 (+) - 瓦伦生产
Chunyang Cao1,2, Haiyan Zhang2, Xuan Cao2
1National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, Liaoning Province Collaborative Innovation Center for Marine Food Deep Processing, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, People's Republic of China.
Journal of agricultural and food chemistry
|July 12, 2023
概括
合成生物学使微生物能够生产有价值的异oprenoids. 研究人员用新的途径对酵母过氧体进行了工程设计,实现了高产的甲 (+) - 烯.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 异烯酸是具有多种生物活性的天然产品.
- 植物提取产生低度的异oprenoids.
- 合成生物学为通过微生物工程生产高价值异oprenoids提供了一个可持续的替代方案.
研究的目的:
- 为了设计和优化酵母过氧体中的异oprenoid生物合成途径.
- 为了增强四烯 (+) - 烯的生产.
主要方法:
- 在酵母过氧体中构建和优化三个不同的异oprenoid 途径 (Haloarchaea 类型,热等离子体类型和异oprenoid 酒精途径).
- 在Haloarchaea-type mevalonate (MVA) 途径中识别速度限制步骤 (MVK和IPK).
- 在摇瓶中进行料批发发酵.
主要成果:
- 与经典的MVA途径相比,Haloarchaea类型的MVA途径在酵母中表现出更高的效率.
- 对Haloarchaea类型MVA通路的优化导致产生869 mg/L的 (+) - 烯.
- 在工程酵母过氧体中成功合成甲 (+) - 烯.
结论:
- 在酵母过氧体中设计的异oprenoid 途径扩大了真核细胞合成能力.
- 开发的Haloarchaea类型的MVA通路为异oprenoid生产提供了更有效的途径.
- 这项研究为微生物合成有价值的异oprenoids建立了一个强大的平台.
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