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尼瓦伦醇在猪卵细胞中介成熟过程中破坏线粒体功能

Jing-Yi Zhang¹, Meng-Yao Zhang¹, Shi-Yi Xiao¹

¹College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China.

Toxicon : official journal of the International Society on Toxinology

|July 12, 2023

概括

尼瓦伦醇毒素暴露显著损害了猪卵细胞的成熟,通过破坏线粒体,增加氧化应激,并导致DNA损伤和亡,最终影响生育能力.

科学领域:

生殖生物学生殖生物学
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

卵细胞成熟对于哺乳动物的生育能力至关重要,影响受精和胚胎发育.
谷物中常见的真菌毒素尼瓦伦醇 (Nivalenol) 具有危险性,包括生殖毒性.
了解尼瓦伦醇对卵细胞的影响对于生殖健康和食品安全至关重要.

研究的目的:

为了研究尼瓦诺对猪卵细胞成熟的影响.
为了阐明尼瓦诺诱导的卵细胞损伤的潜在机制.

主要方法:

猪卵细胞在体外成熟过程中暴露于尼瓦诺.
分析包括对极体挤出,线粒体功能 (分布,膜潜力,ATP产生),水平和氧化应激标志物 (ROS,基因表达) 的评估.
使用Annexin-V和γH2A.X染色评估了细胞亡和DNA损伤.

主要成果:

尼瓦伦醇显著抑制了第一个极体挤出,这是一个关键的成熟指标.
线粒体功能被破坏,以异常分布,减少的膜潜力和减少的ATP生成为证据.
尼瓦伦醇诱导显著的氧化应激 (增加ROS) 并扰乱了相关的基因表达.
在用尼瓦诺治疗的卵细胞中观察到亡和DNA损伤的证据.

结论:

尼瓦伦醇通过多种途径破坏猪卵细胞成熟.
关键机制包括线粒体功能障碍,氧化应激,亡诱导和DNA损伤.
这些发现凸显了尼瓦伦醇对卵细胞质量和潜在的生殖后果的有害影响.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.线粒体中的线粒体.尼瓦伦醇 (Nivalenol) 是一种卵细胞成熟的过程氧化应激是一种氧化应激.

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