从潜在的微藻隔离物中增强氨酸和脂质的混合营养生产 Chlorella sorokiniana C16
Anil Kumar Patel1, Akash Pralhad Vadrale2, Reeta-Rani Singhania1
1Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Centre for Energy and Environmental Sustainability, Lucknow 226 029, Uttar Pradesh, India.
Bioresource technology
|July 12, 2023
概括
研究人员确定了Chlorella sorokiniana C16的高蛋白生产. 优化条件最大限度地提高了蛋白和脂质产量,以实现可持续的生物炼油厂,有利于眼睛健康和生物柴油.
科学领域:
- 生物技术是生物技术.
- 微藻养殖 微藻养殖
- 生物炼油厂计划 生物炼油厂计划
背景情况:
- 氨酸是一种重要的胡卜素,具有显著的健康益处,包括降低视网膜炎和黄斑变性等眼睛疾病的风险.
- 微藻是一种有前途的白蛋白来源,但优化生产以实现商业可行性需要高效的生物工艺.
- 可持续的生物炼油方法可以提高微藻产品提取的经济可行性.
研究的目的:
- 隔离和识别具有高蛋白生产能力的微藻菌株.
- 优化种植参数以最大限度地提高生物质和黄蛋白产量.
- 评估一个可持续的蛋白脂质生物炼油计划的潜力,使用已识别的微藻隔离物.
主要方法:
- 选和隔离高蛋白产生的微藻.
- 优化营养水平 (3X宏观/微量营养),温度 (32°C),光强度 (10k lux) 和盐度 (25%的海水添加).
- 使用分类学方法识别所选的微藻菌株.
主要成果:
- 单独的Chlorella sorokiniana C16表现出高的黄蛋白产量 (71.13 mg/L) 和生物质生产率 (5.2 g/L).
- 在最佳条件下,在32°C时产生高蛋白含量 (17.4 mg/g),在10k lux时产生良好的生长/产量.
- 使用25%海水添加的种植导致显著的脂质积累 (21-27%),适合生物柴油生产.
结论:
- 克洛雷拉索罗基尼亚纳C16是商业化生产黄蛋白的一种非常有前途的菌株,表现出报告中最高的产量之一.
- 优化的生物工艺设计为生物炼油框架内的高价值黄蛋白和脂质联合生产提供了一个可持续的平台.
- 这项研究为开发基于微藻的低成本和可持续的营养药和生物燃料生产系统提供了宝贵的见解.
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