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技术说明:采用贝克曼DXI 800分析仪识别和消除甲状腺功能测试中的生物素干扰的方法,通过利用与Alinity i分析仪的测试协调来利用测试协调

Melissa McBride¹, Amitava Dasgupta²

¹Department of Clinical Laboratory Sciences, University of Kansas Medical Center, Kansas City, KS, USA.

Annals of clinical and laboratory science

|July 12, 2023

概括

使用贝克曼DXI 800分析仪观察到甲状腺功能测试 (TSH,FT3,FT4) 的生物素干扰. 艾利尼蒂i分析仪消除了这种干扰,表明它是准确测量甲状腺激素的可靠替代方案.

科学领域:

临床化学临床化学
免疫测试技术免疫测试技术
内分泌学在内分泌学.

背景情况:

生物素干扰是某些免疫试验中已知的问题,可能导致甲状腺功能测试结果不准确.
贝克曼DXI 800分析仪在自由甲状腺素 (FT4) 和自由三甲状腺素 (FT3) 测定中与显著的生物素干扰有关.
Alinity i分析仪使用TSH,FT3和FT4的非生物素基测定,为生物素干扰提供了潜在的解决方案.

研究的目的:

调查贝克曼DXI 800和Alinity i分析器之间的TSH,FT3和FT4.4的测量协调.
要确定Alinity i分析仪是否消除了DXI 800.所观察到的生物素干扰.
评估Alinity i分析仪的可靠性,以准确测试甲状腺功能.

主要方法:

在DXI 800和Alinity i分析仪上分析了35名患者的TSH,FT3和FT4样本.
准备了一个血清池,并补充了具有不同生物素度的微量.
重新测试补充样本,以评估生物素对两种平台的甲状腺功能测试的影响.

主要成果:

在DXI 800和Alinity i之间观察到TSH,FT3和FT4.4的测试协调.
DXI 800在生物的存在下显示FT3和FT4显著升高,而TSH没有受到影响.
Alinity i分析仪在TSH,FT3或FT4测定中没有证明生物素对生物素的干扰.

结论:

生物素干扰显著影响DXI 800上的FT3和FT4结果,使用Alinity i分析仪解决了这一问题.
Alinity i分析仪提供准确的甲状腺功能测试结果,不受生物素的影响.
在两个平台上,TSH升高与FT3/FT4升高可能表明一种罕见的TSH生成的垂体瘤,与生物素干扰不同.

关键词:

一致性 i 一致性 i 生物素是生物素中的一种.DXI800 DXI800 的意思是什么干扰干扰干扰甲状腺功能测试试验甲状腺功能测试

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