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在非动物实验室哺乳动物Suncus murinus中鉴定了一个syncytin基因
Miki Suzuki1, Akihiro Nakamura2, Yu Matsumoto3
1Department of Reproductive Biology, National Research Institute for Child Health and Development, Tokyo, Japan.
The Journal of veterinary medical science
|July 12, 2023
概括
研究人员在阳胎盘中发现了类似于syncytin-1的蛋白质 (SYN1L1和SYN1L2). 桑库斯SYN1L2蛋白驱动细胞融合,这可能解释了该物种更长的孕期.
科学领域:
- 生殖生物学 生殖生物学
- 分子遗传学 分子遗传学
- 哺乳动物进化 哺乳动物进化
背景情况:
- 合成素 (SYN) 是源自逆转录病毒的内源性蛋白质,对胎盘功能和 trofhoblast 融合至关重要.
- 多核 trofhoblasts 促进胎儿与母亲的血管相互作用.
- 桑库斯·穆林斯 (Suncus murinus) 呈现出更长的怀孕时间 (大约2个月). 30天) 比小鼠,其分子基础目前尚不清楚.
研究的目的:
- 研究Suncus murinus更长的孕期背后的分子机制.
- 在Suncus murinus胎盘中识别和描述syncytin基因和蛋白质.
主要方法:
- 从Suncus murinus胎盘中分离和测序两个假定的syncytin基因 (SYN1L1和SYN1L2).
- 表达载体的构建和引入到培养细胞中以评估蛋白质功能.
- 同类学和结构比较Suncus SYN1L2与相关蛋白质,包括蝙蝠SYN1L.
主要成果:
- 在Suncus murinus胎盘中发现了两个新型的类似于syncytin-1的基因SYN1L1和SYN1L2.
- 在培养细胞中表达时,Suncus SYN1L2蛋白显示出细胞融合活性.
- 桑库斯SYN1L2蛋白与蝙蝠SYN1L具有同质性,并且具有同联蛋白共同的结构特征.
结论:
- 桑库斯SYN1L2是一种功能性syncytin蛋白,能够调解细胞融合.
- 已识别的syncytin蛋白可能在Suncus murinus中观察到的延长妊娠时间中发挥作用.
- 结构上的相似性表明不同哺乳动物物种中syncytin蛋白质的功能性质得到保护.
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