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使用MSFragger-DIA和FragPipe计算平台分析DIA蛋白质组学数据.

Fengchao Yu1, Guo Ci Teo2, Andy T Kong2,3

  • 1Department of Pathology, University of Michigan, Ann Arbor, MI, USA. yufe@umich.edu.

Nature communications
|July 12, 2023
PubMed
概括

我们介绍MSFragger-DIA,这是一个快速而灵敏的方法,用于数据独立采集 (DIA) 质谱 (MS) 蛋白质学中的标识. 这种方法增强了对各种生物样本的定量蛋白质组分析.

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Mass Spectrometry: Molecular Fragmentation Overview01:20

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The ionization of a molecule into a molecular ion inside the mass spectrometer causes instability in the molecule's structure due to the loss of an electron. This eventually leads to the fragmentation or breaking of some bonds in the molecule. The fragmentation occurs predominantly at specific bonds to yield relatively stable fragments.
One type of fragmentation pattern is the cleavage of a single bond in the molecular ion. The cleavage leads to a radical and a cation. The cleavage can...
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