拉曼映射测定TiO2在不同的固体食品样本通过多变量曲线分辨率与交替最小正方形
Priscila Lagner da Silveira Estevão1, Leonardo Francisco Rafael Lemes1, Frederico Luis Felipe Soares2
1Chemistry Department, Federal University of Parana, Curitiba, Parana State, Brazil.
Analytical and bioanalytical chemistry
|July 12, 2023
概括
二氧化 (TiO2),一种常见的食品染料,显示基因毒性. 一种新的拉曼映射和MCR-ALS方法有效地量化了各种食品中的TiO2,而无需复杂的样本准备,有助于监管合规.
科学领域:
- 食品科学与技术 食品科学与技术
- 分析化学 分析化学
- 纳米毒理学研究
背景情况:
- 二氧化 (TiO2),或E171,是一种广泛使用的白色食品染料.
- 对TiO2的基因毒性,特别是其纳米形式的担忧导致了欧盟和其他地方的禁令.
- 有效的TiO2测定方法对于监管监督和消费者安全至关重要.
研究的目的:
- 评估拉曼映射与多变量曲线分辨率交替最小平方 (MCR-ALS) 结合用于在固体食品样本中量化TiO2.
- 开发一种方法,要求对各种食品矩阵进行最小的或不需要任何特定的样品制备.
- 建立一种快速,准确和敏感的分析技术,用于在食品中检测纳米TiO2.
主要方法:
- 拉曼映射用于从食品样本中获取光谱数据.
- 多变量曲线分辨率交替最小平方 (MCR-ALS) 用于光谱数据分析和TiO2量化.
- 该方法应用于各种不同成分的固体食品样本,没有进行特定的预处理.
主要成果:
- 提出的方法成功量化了TiO2在不同的食品矩阵在没有特定的样本制备.
- 该技术显示了高精度 (93%的恢复),低检测 (0.0111%m/m) 和量化 (0.0370%m/m) 的极限.
- 实现了优异的线性 (r=0.9990) 和精度 (标准偏差~0.020-0.030%w/w),突出了该方法的稳定性.
结论:
- 拉曼映射与MCR-ALS相结合,为食品中纳米-TiO2的确定提供了一个强大的,多功能工具.
- 这种方法消除了对大量样本准备的需要,简化了对各种食品类型的分析.
- 该技术显示出在商用食品中的TiO2常规质量控制和监管监测方面具有重大潜力.
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