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通过使用RNA测序来探索暴露在人类桃体细胞中的金黄色葡萄球菌 (Staphylococcus aureus) 的差异表达基因

Srijana Bastakoti¹, Clement Ajayi^2,3, Kjersti Julin²

¹Department of Medical Biology, Host-Microbe Interaction (HMI) research group, UiT - The Arctic University of Norway, Tromsø, Norway. srijana.bastakoti@uit.no.

BMC microbiology

|July 12, 2023

概括

这项研究通过分析暴露于人类桃体细胞时的基因表达变化来研究黄金葡萄球菌 (S. aureus) 的喉殖民. 研究人员确定了参与粘附和免疫逃避的关键基因,为预防喉感染提供了潜在的目标.

科学领域:

微生物学微生物学
基因组学就是基因组学.
传染性疾病传染性疾病

背景情况:

黄金葡萄球菌通常会殖民于鼻子和喉,造成感染的风险.
与鼻腔殖民相比,对S. aureus喉殖民的理解有限.

研究的目的:

探索S. aureus在与人类桃体上皮细胞 (HTEpiC) 相互作用期间的差异表达基因 (DEG).
为了分析S. aureus的转录组概况,以应对宿主细胞随时间的暴露.

主要方法:

使用RNA测序 (RNA-seq) 来识别S. aureus TR145.5中的DEGs.
共同培养实验使用S. aureus和HTEpiC进行,1小时和3小时的间隔.
反转录定量PCR (RT-qPCR) 用于验证RNA-seq发现.

主要成果:

在暴露于HTEpiC.后,在S. aureus中共发现了508种DEG.
参与细菌粘附,免疫逃避和铁获取/运输的基因是DEGs之一.
基因表达模式在1小时到3小时的暴露时间之间有所不同,其中一些是共享的,一些是独特的DEGs.

结论:

人类桃体上皮细胞 (HTEpiC) 作为一个合适的体外模型,用于研究黄金菌与宿主相互作用.
已识别的DEGs,特别是那些在宿主细胞接触时诱导的DEGs,代表了治疗干预的潜在目标.
对这些DEG的进一步研究可能会导致预防S. aureus殖民和喉感染的策略.

关键词:

人类桃体上皮细胞有关RNA测序的RNA测序黄金葡萄球菌黄金葡萄球菌喉殖民化的殖民化文字转录学 (Transcriptomics) 是一个学科.

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