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相关概念视频

Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

12.7K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
12.7K
Overview of DNA Repair02:25

Overview of DNA Repair

31.1K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
31.1K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

10.0K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
10.0K
Homologous Recombination02:31

Homologous Recombination

50.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.7K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.1K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
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Mismatch Repair01:36

Mismatch Repair

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Overview
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相关实验视频

Updated: Jul 23, 2025

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

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模板插入-DNA修复变得很有特技.

Susanna Stroik1, Adam J Luthman2, Dale A Ramsden1,2,3

  • 1Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

Environmental and molecular mutagenesis
|July 13, 2023
PubMed
概括
此摘要是机器生成的。

通过聚合酶甲基 (Polθ) 来修复DNA双链断裂可以导致删除和插入. 这项研究发现了一种新的"链切换"插入机制,有助于癌症等疾病.

关键词:
在DNA双链断裂修复中.这是一个很好的创业公司.癌症 癌症 癌症 癌症 癌症聚合酶乙烯基酶是什么?

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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

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Visualization of DNA Repair Proteins Interaction by Immunofluorescence
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Visualization of DNA Repair Proteins Interaction by Immunofluorescence

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相关实验视频

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Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
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Visualization of DNA Repair Proteins Interaction by Immunofluorescence
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 基因组学就是基因组学.

背景情况:

  • 通过各种机制来修复DNA双链断裂 (DSBs),包括theta介导末端连接 (TMEJ).
  • 众所周知,TMEJ通过微同质 (MH) 调整引起删除.
  • 此外,TMEJ还具有模板插入功能,不太了解并与删除和插入相关.

研究的目的:

  • 为了研究聚合酶甲基 (Polθ) 中介模板插入的机制.
  • 描述一种新发现的类型的Polθ-依赖插入称为"链切换".

主要方法:

  • 对DNA修复途径的分析,特别关注聚合酶甲基.
  • 在多种物种和位置上识别和描述直接,反向和链切换模板插入.

主要成果:

  • 聚合酶甲介于模板插入,通过多步合成过程.
  • 确定了一种新的模板插入类型,即"链切换",并观察到显著的频率.
  • 这些依赖Polθ的插入经常在癌症基因组和重复扩张障碍中发现.

结论:

  • 聚合酶甲基介导的模板插入,包括新发现的链切换类,有助于突变发生.
  • 这些机制与癌症和重复扩张障碍等疾病的发病有关.
  • 了解Polθ插入机制为新的治疗策略提供了潜力.