相关实验视频

Updated: Jul 23, 2025

Modeling Fetal Alcohol Spectrum Disorders in Zebrafish to Characterize the Impact of an Adverse Embryonic Environment on Adult Social Behavior

Modeling Fetal Alcohol Spectrum Disorders in Zebrafish to Characterize the Impact of an Adverse Embryonic Environment on Adult Social Behavior

Published on: February 9, 2024

[突出的信号通路抑制剂DAPT改善了斑马鱼中酒精诱导的神经元分化障碍]

G Yin¹, R Li², Y Liu³

¹Medical Research Center of Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Nan fang yi ke da xue xue bao = Journal of Southern Medical University

|July 13, 2023

概括

斑马鱼暴露于乙醇会通过调节Notch信号通路来破坏神经元发育和感觉运动技能. 用DAPT抑制这种途径可以减轻这些有害影响,提供潜在的治疗见解.

科学领域:

神经科学是一个神经科学.
发展生物学发展生物学
遗传学是一个遗传学.

背景情况:

胎儿酒精谱系障碍 (FASD) 是可预防的智力障碍的主要原因.
诺奇信号通路在胚胎发育中起着至关重要的作用,包括神经元分化.
了解FASD背后的分子机制对于开发有效干预措施至关重要.

研究的目的:

研究Notch信号通路在FASD斑马鱼模型中神经元分化和感觉运动功能的作用.
为了确定抑制Notch信号通路是否可以改善乙醇诱导的发育缺陷.

主要方法:

斑马鱼胚胎暴露于乙醇和/或DAPT,一个Notch抑制剂.
评估了死亡率,化,形率和身体长度.
关键神经元分化标记物 (sox2,neurogenin1,huc) 和Notch通路基因的mRNA表达被使用qRT-PCR和现场杂交分析.
通过对光线和振动刺激的反应来评估感觉运动行为.

主要成果:

单独使用DAPT治疗增加了死亡率和形率,同时降低了化率和身体长度.
DAPT改变了神经元标记的mRNA表达,降低了sox2的调节,并提高了神经原蛋白1和huc的调节.
乙醇暴露上调了诺奇通路基因 (notch1a,her8a,NICD).
联合乙醇和DAPT治疗部分扭转了乙醇诱导的神经元分化标志物的缺陷,并改善了感觉运动反应.

结论:

乙醇暴露会破坏斑马鱼的神经元分化和感觉运动能力,部分是通过Notch通路的上调调节.
抑制Notch信号通路可以减轻乙醇暴露对斑马鱼发育的有害影响.
准Notch路径可能为FASD提供治疗策略.

关键词:

DAPT DAPT 是一个应用程序.缺口信号通道的信号通道.乙醇乙醇是一种胎儿酒精谱系障碍胎儿酒精谱系障碍神经元分化神经元的分化

更多相关视频

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure

Assessing Teratogenic Changes in a Zebrafish Model of Fetal Alcohol Exposure

Published on: March 20, 2012

Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography

Published on: February 11, 2020

相关概念视频

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...