UMAD1在细胞因子解离过程中对ESCRT-III动态子单元的转换有所贡献
James Glover1, Edward J Scourfield1, Leandro N Ventimiglia1
1Department of Infectious Diseases, King's College London, Faculty of Life Sciences & Medicine, London SE1 9RT, UK.
Journal of cell science
|July 13, 2023
概括
一个新的ESCRT-I子单元UMAD1,通过促进子细胞的最终分离,对细胞分裂 (细胞动力学) 起到至关重要的作用. 它连接ESCRT-I和ESCRT-III,确保细胞分裂期间适当的中体脱离.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 细胞动力学,细胞分裂的过程,以脱离结论,其中中体细胞间桥梁被解决.
- 运输 (ESCRT) 机器所需的内体组分复合体调解了这一关键的膜重塑事件.
- CEP55作为支架,招募早期的ESCRT因素,如ESCRT-I和ALIX,通过ESCRT-III聚合物启动中体切断.
研究的目的:
- 识别参与细胞运动的新型因素,并了解它们在中体脱离中的作用.
- 阐明UMAD1在细胞分裂过程中的ESCRT通路中的功能.
- 研究ESCRT-I和ESCRT-III在细胞动因学的最后阶段之间的相互作用.
主要方法:
- 同免疫沉测试以确定蛋白质相互作用.
- 使用siRNA评估蛋白质功能的耗尽研究.
- 活细胞成像用于观察细胞动力学过程中的中体动态.
- 免疫光显微镜用于分析蛋白质定位.
主要成果:
- 鉴定出UMAD1是一种ESCRT-I子单元,可选择性地与VPS37C和VPS37B结合,促进细胞动力学特异性的ESCRT-I组合.
- TSG101将UMAD1招募到中体,稳定CEP55-ESCRT-I的相互作用,并促进脱离.
- UMAD1和ALIX都对中体ESCRT-III子单元的动态交换至关重要,这一过程对于高效的脱离至关重要.
结论:
- UMAD1是细胞运动的关键调节剂,是ESCRT-I和ESCRT-III之间的重要联系.
- 在最后的脱离阶段,UMAD1-ESCRT-I相互作用是不可或缺的,确保了适当的细胞分离.
- 在调节ESCRT-III动态方面UMAD1的作用突出显示了一种新的机制,这对于成功的细胞动力学至关重要.
更多相关视频
相关概念视频
Intralumenal Vesicles and Multivesicular Bodies
3.6K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
3.6K
Cell Motility through Blebbing
2.0K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
2.0K
The Spindle Assembly Checkpoint
3.2K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.2K
Separation of Sister Chromatids
3.7K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
At the onset of anaphase, separase, a proteolytic enzyme, is...
3.7K
Anaphase Promoting Complex
2.9K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.9K
The Unfolded Protein Response
4.8K
The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
4.8K


