通过热蛋白质组剖析进行药物标识的定量质谱方法的比较
Amy L George1, Frances R Sidgwick1, Jessica E Watt2
1Laboratory for Biological Mass Spectrometry, Biosciences Institute, Newcastle University, Newcastle-upon-Tyne NE2 4HH, U.K.
Journal of proteome research
|July 13, 2023
概括
数据独立获取 (DIA) 为热蛋白质组概况 (TPP) 提供了一个具有成本效益的替代方案. 无标签的DIA方法准确地检测药物向参与,类似于TMT标记的数据依赖获取 (DDA).
科学领域:
- 蛋白质组学是指蛋白质组学.
- 化学生物学 化学生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 热蛋白质组概况 (TPP) 对于识别药物向相互作用至关重要.
- 准确的量化对于检测目标接触时的热稳定性变化至关重要.
- 与数据依赖获取 (DDA) 相关的同质双重质量标签 (TMT) 提高了精度,但也有局限性.
研究的目的:
- 评估无标签数据独立获取 (DIA) 方法用于TPP中的热移量化.
- 为了比较DIA性能与TMT标记的DDA,用于检测药物向参与.
- 评估DIA在TPP工作流程中的成本效益.
主要方法:
- 探索了各种无标签的DIA量化策略.
- 使用了用MAPK14抑制剂losmapimod治疗的急性髓性白血病细胞.
- 将DIA-NN (无图书馆模式) 的性能与TMT-DDA进行比较.
主要成果:
- 没有标签的DIA方法在热移量测量中显示出与TMT-DDA相似的性能.
- 美国国防情报局成功检测到洛斯马皮莫德与其目标MAPK14和MAPKAPK3.3的接触.
- 特别有效的是DIA-NN的无图书馆模式.
结论:
- 无标签的DIA是TPP的可行和经济有效的替代品.
- 在TPP中,DIA提供了药物向相互作用的准确检测.
- 这种方法简化了TPP的工作流程并降低了成本.
关键词:
急性髓性白血病 (AML) 是一种疾病.数据依赖的获取 (DDA)数据独立获取 (DIA) 是指数据独立获取.混合图书馆 混合图书馆 混合图书馆频谱图书馆是一个光谱图书馆.双重质量标签 (TMT) 是一种双重质量标签.目标是去除卷积.热性蛋白质组概况 (TPP) 分析更多相关视频
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