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绘制怀孕依赖的硫水揭示了人类子宫动脉中蛋白质硫水的各种功能

Jin Bai¹, Fenglong Jiao², Alejandra Garcia Salmeron¹

¹Department of Obstetrics and Gynecology, University of California, Irvine, CA 92697, USA.

|July 13, 2023

概括

硫化 (H2S) 在怀孕期间显著增加子宫动脉中的蛋白质硫化,影响血管光滑肌功能和子宫血流调节. 这项研究揭示了H2S在管理怀孕血液动力学方面的新角色.

科学领域:

生理学生理学生理学
生物化学生物化学
蛋白质组学是指蛋白质组学.

背景情况:

硫化 (H2S) 产量在怀孕期间增加,并导致子宫血管扩张.
通过H2S影响子宫血液动力学的精确机制在很大程度上是未知的.
蛋白质s-硫化是H2S发挥其功能作用的主要途径.

研究的目的:

量化子宫动脉硫水组 (硫化蛋白) 对H2S的反应中的怀孕相关变化.
在怀孕和非怀孕的人类子宫动脉中识别H2S修饰的蛋白质上的特定的囊蛋白位.
阐明蛋白质硫化在妊娠期间调节子宫血动力学中的作用.

主要方法:

利用Tag-Switch试验量化人类子宫动脉中的硫化蛋白质.
采用低pH的定量醇反应性概况,加上液体染色学-双重质谱法.
进行生物信息学分析和免疫阻塞,以验证发现并确定特定的蛋白质修饰.

主要成果:

孕妇的总硫化蛋白水平明显高于非孕妇的子宫动脉.
确定了数百种H2S反应的硫化和蛋白质,特别是那些参与血管光滑肌肉收缩和放松的蛋白质.
尽管KATP通道总水平没有变化,但发现了硫化KATP通道的妊娠依赖性上调.

结论:

蛋白硫化在妊娠期间调节子宫动脉血动力学方面发挥着至关重要的作用.
内源和外源的H2S显著影响子宫动脉硫.
这些发现揭示了蛋白质硫化在管理妊娠特异性血管适应方面的新功能.

关键词:

在H2S信号传输中.功能性的蛋白质组学怀孕怀孕怀孕怀孕怀孕硫化化硫化化子宫动脉的子宫动脉.

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