mRNA分离激活剂 Pat1 和 Dhh1 调节了转录的丰富性和翻译性,以调整细胞对营养可用性的反应
Anil Kumar Vijjamarri1, Neha Gupta1, Chisom Onu2
1Division of Molecular and Cellular Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Nucleic acids research
|July 13, 2023
概括
酵母蛋白Pat1和Dhh1抑制了特定mRNA的翻译和丰富性. 这些因素的损失会损害mRNA的循环和转录,在富含营养的条件下激活营养有限的途径.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 转录后条例 转录后条例 转录后条例
背景情况:
- 在酵母中,Pat1和Dh1是已知的mRNA分解激活剂.
- 它们在营养补充条件下抑制翻译和mRNA丰富性的特定作用需要进一步阐明.
研究的目的:
- 研究Pat1和Dhh1在控制特定mRNA转化和丰度方面的功能.
- 确定这些因素对mRNA循环和转录的影响.
- 了解它们在抑制通常在营养限制期间激活的途径中的作用.
主要方法:
- 核糖体造型分析 核糖体造型分析
- 在RNA-Seqq.
- 封闭的mRNAs的CAGE分析
- 在RNA聚合酶IIChIP-Seqq中.
- TMT - 质谱测量 - 质谱测量
- 对缺乏Pat1和/或Dhh1的酵母突变的分析.
主要成果:
- 数百个非环境应激反应 (ESR) 转录在dhh1Δ和pat1Δ突变中升高,表明野生类型细胞中的Pat1/Dhh1的累积抑制.
- 损坏的mRNA循环 (减少分离和减少转录) 驱动突变体中的转录失效.
- 帕特1和DHH1合作抑制细胞粘附,艾伦因利用,呼吸和自的mRNA的翻译和蛋白质生产.
- 在突变者中观察到呼吸和自的升高.
结论:
- Pat1 和 Dhh1 作为后转录抑制剂,在营养补充条件下阻止营养限制通路的激活.
- 它们的功能通过控制mRNA稳定性和翻译来维持细胞平衡至关重要.
- 这些因素共同调节了一系列广泛的转录,影响各种代谢和细胞过程.
相关概念视频
Translational Regulation
45
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
45
Riboswitches
8.2K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.2K
Regulation of Expression Occurs at Multiple Steps
22.8K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.8K
Regulation of Expression at Multiple Steps
945
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
945
mRNA Stability and Gene Expression
5.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
5.6K
Stringent Response in E. coli
31
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
31


