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沙利化物通过STAT3/HIF-1α/RORγt通路影响无形成性贫血中Th17/Treg细胞平衡

Zenghui Liu^1,2, Lixuan Chen¹, Defang Xiong³

¹Guangzhou University of Chinese Medicine, Guangzhou, People's Republic of China.

Redox report : communications in free radical research

|July 13, 2023

概括

来自Rhodiola rosea的salidroside通过恢复T细胞平衡来治疗获得的无形成性贫血 (AA) 是有前途的. 这项研究揭示了它的机制涉及调节STAT3/HIF-1α/RORγt通路.

科学领域:

免疫学免疫学免疫学
血液学血液学血液学
药理学药理学是指药理学的学科.

背景情况:

获得的无塑性贫血 (AA) 是一种严重的疾病,与不平衡的Th17/Treg细胞比率有关.
恢复Th17/Treg细胞平衡是一种潜在的AA治疗策略.
罗迪奥拉在AA中表现出治疗效果,但其潜在的机制需要阐明.

研究的目的:

为了研究沙利德化物 (Rhodiola rosea的关键成分) 对成年AA患者的Th17/Treg细胞平衡的影响以及相关的小鼠模型.
在AA的背景下,探索沙利德化物影响的分子途径.

主要方法:

在AA患者的周围血液中量化HIF-1αmRNA和蛋白质.
使用流式细胞计,qRT-PCR和西式斑点测试来评估沙利德对T细胞分化,Th17/Treg细胞和关键信号分子 (STAT3,HIF-1α,RORγt) 的影响.
用ELISA测量小鼠血清中的造血生长因子.

主要成果:

在AA患者中,HIF-1α水平升高.
在小鼠中,沙利多胺的使用增强了造血功能,增加了血液细胞数量,并改善了骨髓细胞性.
沙利德化物调节的细胞因子概况,诱导SCF,TPO和IL-3,同时抑制IL-2.
沙利德化物治疗导致STAT3,HIF-1α,RORγt和IL-17a的表达减少,并伴随着FoxP3表达的增加,从而纠正了Th17/Treg失衡,无论是*in vitro*还是*in vivo*.

结论:

沙利德化物显示出作为一种新型治疗药物的潜力,用于获得的无可塑性贫血.
沙利多在AA中的治疗效果是通过纠正Th17/Treg细胞失衡来调节的.
沙利多的作用机制涉及到STAT3/HIF-1α/RORγt信号通路的调制.

关键词:

在STAT3/HIF-1α/RORγt路径中.沙利德化物是一种化物.Th17/Treg 余额的情况没有无形性贫血.

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