相关实验视频

Updated: Jul 23, 2025

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

在模型中进行核糖体 Thalassiosira 伪称的核糖体剖析

Monica Pichler^1,2, Andreas Meindl^3,2, Markus Romberger³

¹School of Environmental Sciences, University of East Anglia, Norwich, United Kingdom.

Current protocols

|July 13, 2023

概括

研究人员开发了一种用于藻类的新型核糖体分析协议. 这种方法可以对这些重要的海洋微藻中的蛋白质合成和翻译调节进行全球分析.

科学领域:

海洋生物学海洋生物学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

藻是关键的真核微藻,参与海洋生态化学循环.
它们具有显著的生物技术潜力,但它们的转化调节机制尚不清楚.

研究的目的:

开发和详细介绍一个核糖体分析协议,用于研究模型藻Thalassiosira pseudonana的翻译.
为其他藻物种提供一种可适应的方法来研究蛋白质合成调节.

主要方法:

用糖糖超离心和PAGE来分离由核糖体保护的mRNA片段.
通过与生物化寡头的减去性杂交,减少了rRNA污染.
碎片被转化为测序库,用于全球蛋白质合成分析.

主要成果:

对Thalassiosira pseudonana.成功建立了一个强大的核糖体分析协议.
该协议允许量化和分析藻中蛋白质合成变化的情况.
这种方法显著增强了藻研究的分子工具包.

结论:

开发的协议推进了对藻中翻译调节的研究.
这项研究为了解浮游植物生物学和生物技术开辟了新的途径.
该协议易于适应各种藻物种,促进更广泛的研究应用.

关键词:

这是一个RNARNARNARNARNA.泰拉西奥西拉是一个假名.藻类是一种藻类.高通量测序的高通量测序核糖体造型分析是指核糖体造型.翻译翻译翻译翻译翻译翻译

更多相关视频

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

Eukaryotic Polyribosome Profile Analysis

Published on: June 15, 2010

相关概念视频

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

Ribosomes

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...