重症监护病房的水槽一直被多种耐药细菌和可调动的,传递耐药性的塑菌殖民
Luke Diorio-Toth1, Meghan A Wallace2, Christopher W Farnsworth2
1The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine , St. Louis, Missouri, USA.
mSystems
|July 13, 2023
概括
医院的水槽中存在多重耐药生物 (MDROs),基因组分析揭示了长期污染和抗生素耐药基因 (ARGs) 通过等离子体的传播. 积极监测建筑环境对于缓解MDRO传播至关重要.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 医院水槽是微生物病原体的潜在储存库,对患者构成风险.
- 了解水槽中多药耐药生物体 (MDROs) 的殖民动态至关重要,特别是在重症监护室 (ICU).
研究的目的:
- 在ICU水槽和水中调查MDRO的长期殖民模式.
- 分析抗生素耐药性 (AR) 在这些环境中维持和传播的机制.
主要方法:
- 未来27个月内从两个医院收集ICU水槽和水的样本.
- 基于培养的细菌物种隔离和全基因组测序 (包括纳米孔测序).
- 对822个细菌分离物的基因组分析,代表了104个物种/基因组.
主要成果:
- 鉴定了长期殖民的 * 伪 * spp. 在ICU水槽中使用的Serratia marcescens.
- 在无关的细菌宿主中显示出抗性传递等离子体的持久性.
- 与社区样本相比,在ICU相关的细菌中发现抗生素耐药性基因 (ARG) 的流行率更高.
- 证明了在 *Pseudomonas* spp. 中的 AR. 是通过菌株殖民和水平基因转移 (HGT) 维持的,而HGT 独立地在 *Acinetobacter* spp.中维持 AR. 和肠杆菌类.
结论:
- 医院的水槽作为MDROs的持续储存库.
- 横向的基因转移,特别是通过等离子体,在抗生素耐药性的传播中起着重要作用.
- 对建筑环境的基因组集中监测对于医疗保健机构有效的MDRO控制至关重要.
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