生物火联合感染小组对联合感染管理的性能和假设影响:回顾性分析
Benjamin Berinson1, Laura Spenke1, Lukas Krivec2
1Institute for Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Journal of clinical microbiology
|July 13, 2023
概括
生物火联合感染试验 (BJA) 准确地识别了性关节炎的病原体,比传统培养提供更快的结果. 这种分子测试显示出高灵敏度和特异性,有可能改善患者管理和抗生素治疗.
科学领域:
- 微生物学 微生物学
- 分子诊断学 分子诊断学
- 传染性疾病 传染性疾病
背景情况:
- 败血性关节炎的诊断依赖于病原体的识别,通常受到培养限制的阻碍.
- 抗生素治疗前和难以培养的生物挑战了标准的诊断方法.
研究的目的:
- 评估BioFire关节感染试验 (BJA) 与败血性关节炎标准培养基诊断的性能.
- 评估BJA对患者管理和抗生素治疗优化的潜在影响.
主要方法:
- 使用多重PCR面板 (BJA) 分析了120名患者的123个突液样本.
- 比较BJA结果与基于培养的诊断 (标准护理[SOC]).
- 收集了临床数据,以评估对患者管理的影响.
主要成果:
- 在15种培养阳性病原体中,BJA正确识别了9种,在培养阴性病例中检测到4种额外的病原体.
- 对于面板上的生物体,BJA表现出100%的灵敏度和100%的特异性.
- 与SOC相比,BJA提供了显著更快的物种识别 (49小时) 和分子敏感性数据 (99小时).
结论:
- BJA是一种敏感和特定的分子工具,用于检测细菌性关节炎中普遍存在的病原体.
- 来自BJA的更快的结果可以实现更及时,更优化的抗生素治疗.
- 由于遗漏的病原体,如凝结酶阴性葡萄球菌和Cutibacterium acnes等,对假肢关节感染的测试的实用性是有限的.
相关概念视频
Antimicrobial Effectiveness
50
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
50
Factors Affecting the Risk of Infection
12.0K
The hosts' susceptibility to infection depends on several factors. The integrity of the skin and mucous membranes helps protect the body against microbial attacks. When the skin is altered, the chance of infection, limb loss, and even death increases.
The integrity and count of the white blood cells help the body resist pathogens and fight infection. When impaired, it reduces the body's resistance to pathogens. The acidic pH levels of the gastrointestinal, genitourinary tracts, and skin...
The integrity and count of the white blood cells help the body resist pathogens and fight infection. When impaired, it reduces the body's resistance to pathogens. The acidic pH levels of the gastrointestinal, genitourinary tracts, and skin...
12.0K
Acute Pyelonephritis II: Diagnostic Studies and Management
17
Introduction:For diagnosing acute pyelonephritis, a comprehensive patient history is collected to identify symptoms such as dysuria, frequent or urgent urination, flank pain, or costovertebral angle (CVA) tenderness that may suggest a kidney infection.Physical ExaminationDuring the physical examination, CVA tenderness is assessed. This involves gentle percussion over the costovertebral angle, where tenderness often indicates a kidney infection.Diagnostic TestsUrinalysis: Used to identify white...
17
Endocarditis III: Medical Management
8
Infective endocarditis management involves a multifaceted approach encompassing infection prevention, lifestyle modifications, pharmacological therapy, and surgical management.Infection Prevention:Hand Hygiene: Thorough handwashing is crucial to prevent the spread of infection. Hand hygiene should be performed regularly, especially before and after using the restroom.Oral Hygiene: Good oral hygiene is essential. It includes brushing teeth immediately after waking up and before bed, flossing...
8
Healthcare Associated Infections II: Preventive Measures
2.7K
Essential infection prevention measures are based on the knowledge of the infection chain, the modes of transmission in healthcare settings, and the use of the best practices in all healthcare settings. Compulsory public reporting of healthcare-associated infection rates is needed to allow individuals and the community to make informed choices regarding selecting a healthcare facility.
The best practices for preventing healthcare-associated infections include hand hygiene, patient risk...
The best practices for preventing healthcare-associated infections include hand hygiene, patient risk...
2.7K
Urinary Tract Infection III: Diagnostic Studies and Interprofessional Care
15
A healthcare provider can diagnose a urinary tract infection (UTI) through several methods:Medical History and Symptoms: The provider will take a detailed medical history and ask about symptoms such as frequent urination, burning sensation during urination, and lower abdominal pain.Urinalysis: A clean-catch urine sample is collected in a sterile container and tested for the presence of bacteria, white blood cells (leukocytes), nitrites, blood, and protein. The presence of leukocytes and...
15


