从15,000多名患者的血微生物无细胞DNA测序确定了广泛的病原体谱
Sarah Y Park1, Eliza J Chang1, Nathan Ledeboer2
1Karius, Redwood City, California, USA.
Journal of clinical microbiology
|July 13, 2023
概括
微生物无细胞DNA (mcfDNA) 测序提供了公正的病原体检测. 这项大规模的研究证明了其临床实用性,在超过15,000名患者中识别了各种微生物,并实现了快速的周转时间.
科学领域:
- 临床微生物学 临床微生物学
- 基因组诊断 基因组诊断 基因组诊断
- 传染病流行病学 传染病流行病学
背景情况:
- 微生物无细胞DNA (mcfDNA) 测序是传染病的新兴诊断工具.
- 卡里乌斯测试是一种商业测试,可检测和量化血中的mcfDNA.
研究的目的:
- 为了评估实验室质量指标,报告的病原体,以及用血mcfDNA测序测试的大量患者队列的临床数据.
- 评估通过这种规模的诊断方法识别的微生物病原体的范围和复杂性.
主要方法:
- 分析了来自美国39个州的15165名患者的18690份商业测试报告 (2018年4月至2021年9月).
- 从要求表格评估实验室质量指标,病原体检测和患者临床数据.
- 专注于在规模上进行的临床级的元基因组测试.
主要成果:
- 平均周转时间为26小时,测试结果有效率为96%.
- 58%的报告至少确定了一种分类,检测到701种独特的微生物分类.
- 通常检测到的病原体包括细菌,病毒和真菌,特别关注机会性和具有诊断挑战性的微生物.
结论:
- 这项研究代表了在临床环境中进行血mcfDNA测序的最大报告队列.
- 这些发现突显了该测试的可扩展性,并为在不同临床场景中识别的病原体谱提供了新的见解.
- 血mcfDNA测序是无偏,广泛的病原体检测和量化的一个有价值的工具.
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