追踪B细胞对SARS-CoV-2mRNA-1273疫苗的反应
Felipe Lopes de Assis1, Kenneth B Hoehn2, Xiaozhen Zhang1
1Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Cell reports
|July 13, 2023
概括
接种SARS-CoV-2mRNA-1273疫苗会产生长期存储的B细胞 (MBCs),这些B细胞可预测地进化. 多模式单细胞分析揭示了这些关键的疫苗诱导免疫细胞中的克隆扩张和突变积累.
科学领域:
- 免疫学 免疫学 免疫学
- 疫苗学 疫苗学 疫苗学
- 一个单细胞分析.
背景情况:
- 保护性免疫依赖于长寿的抗体分泌细胞和记忆B细胞 (MBCs).
- 了解SARS-CoV-2 mRNA疫苗反应的动态对于公共卫生至关重要.
研究的目的:
- 为了纵向评估对两剂SARS-CoV-2 mRNA-1273疫苗的免疫反应.
- 用多式单细胞分析来描述接种疫苗后B细胞的演变.
主要方法:
- 多模式单细胞分析,包括表面蛋白质,转录组学和B细胞受体 (BCR) 谱系分析.
- 疫苗接种后从未感染过的个体中长度取样免疫细胞.
- 排序和分析等离子体细胞和尖端蛋白阳性 (S-2P+) 和阴性 (S-2P-) B细胞.
主要成果:
- 在S-2P+B细胞中观察到克隆扩张和累积突变.
- 这些细胞富含免疫球蛋白G表达型MBCs,并遵循了分开的进化路径.
- 在这个轨迹上确定了不同的B细胞子集,包括CD11c+非典型的MBC和CD71+激活的前体.
- 在队列内和队列之间观察到BCR融合,这表明了协调的B细胞进化.
结论:
- 接种SARS-CoV-2mRNA疫苗会诱导可预测和协调的记忆B细胞进化.
- 鉴定的B细胞轨迹为长期保护性免疫的产生提供了洞察力.
- 多模式单细胞分析是一种强大的方法,用于剖析疫苗诱导的免疫反应.
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