感冒病例:重新评估CRISPR/Cas系统在Mycobacteriaceae中发生的情况
Evan Brenner1, Srinand Sreevatsan1
1Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI, United States.
Frontiers in microbiology
|July 13, 2023
概括
克里斯普尔/卡斯系统在菌根菌中很常见,这挑战了它们作为进化标记物的使用. 这些系统,包括III-A型,在物种中广泛存在,表明Mycobacteriaceae家族中频繁的基因转移和进化.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 进化生物学 进化生物学
背景情况:
- 细菌的CRISPR/Cas系统对于对抗外来DNA和基因调节的防御至关重要.
- 克里斯普尔/卡斯系统已被用于研究*Mycobacterium结核病*复合体 (MTBC) 的演变.
- 关于非结核性菌根 (NTM) 中的CRISPR/Cas系统的知识有限.
研究的目的:
- 调查NTM中CRISPR/Cas loci的流行和多样性.
- 了解*Mycobacteriaceae*家族中的CRISPR/Cas系统的进化历史.
- 评估 III-A 类型 Cas 系统作为 MTBC 演变标记物的可靠性.
主要方法:
- 现有的真菌细菌基因组的生物信息分析.
- 对CRISPR/Cas loci.的遗传学分析
- 在各种NTM物种中识别Cas系统.
主要成果:
- 克里斯普尔/卡斯系统在*Mycobacteriaceae*中广泛存在,有些物种拥有多种类型.
- 卡斯系统在NTM中表现出分散的分布,经常出现收益和损失.
- 在*Mycobacterium heckeshornense*和*Mycobacterium* SM1中发现了III-A型Cas系统,不仅在MTBC中.
结论:
- 菌根的CRISPR/Cas系统的数量和多样性比以前想象的要多.
- 类型III-A Cas系统不是MTBC进化研究的可靠标记.
- 水平基因转移是菌根CRISPR/Cas系统中遗传变异的重要驱动因素.
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