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Updated: Jul 23, 2025

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在小鼠器官生成过程中对细胞状态特定的基因调节程序的单细胞色素可访问性概况

Qiuting Deng^1,2, Shengpeng Wang^1,2, Zijie Huang^1,3

¹College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China.

Frontiers in neuroscience

|July 13, 2023

概括

这项研究使用单细胞ATAC-seq绘制了小鼠早期胚胎中的染色质地图,揭示了器官生成的关键因素,并确定了与人类发育障碍的潜在联系.

科学领域:

发展生物学发展生物学
基因组学就是基因组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

在哺乳动物中,早期的器官生成遵循胃化,涉及原生细胞的特异化.
了解动态色素格局对于破译发育机制至关重要.

研究的目的:

在小鼠早期器官生成过程中研究前体细胞的动态色素景观.
通过单细胞表观遗传学来确定推动胚胎发育的分子机制.

主要方法:

在小鼠胚胎 (E8.5-E10.5) 上使用测序 (scATAC-seq) 进行了转化酶可访问性染色质的单细胞试验.
集成的scATAC-seq数据与公开的单细胞RNA测序 (scRNA-seq) 数据.
确定了参与脊髓和体质生成的 cis 调节元件和转录因子.

主要成果:

分析了101599个单细胞,确定了41种不同的细胞类型.
发现了关键的 cis 调节元件和转录因子驱动脊髓和体质生成.
通过与疾病位置交叉可访问的峰值来识别潜在的临床相关单核酸变体 (SNV).

结论:

为了解植入后发育中的细胞命运决定提供了基础资源.
阐明了早期有机体发生的基础分子机制.
扩大了关于人类发育形病理学的知识.

关键词:

这些是cis-regulatory元素.鼠标的器官发生.抛向性质的中皮层.一个单细胞的ATAC-seqq.脊髓是指脊髓的部分.

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