鉴定了一种表达功能性编程死亡受体-1 的新型带血NK细胞亚群
Marco Greppi1, Valentina Obino1, Rayan Goda1
1Department of Experimental Medicine, University of Genoa, Genoa, Italy.
Frontiers in immunology
|July 13, 2023
概括
编程细胞死亡蛋白1 (PD-1) 在新生儿的带血上表达自然杀手细胞 (NKs),独立于母亲的细胞巨乳病毒 (CMV) 感染. 在NK细胞上这种功能性PD-1表达为免疫疗法治疗提供了潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 自然杀手 (NK) 细胞对于天生的免疫力至关重要,表现出强大的抗瘤和抗病毒活性.
- 编程细胞死亡蛋白1 (PD-1) 被确定为成人终端分化NK细胞的抑制受体,特别是那些先前感染了细胞巨乳病毒 (CMV) 的人.
- 在NK细胞上PD-1的存在和发育调节,特别是与CMV相关的存在和发育调节,在围产阶段的环境中仍然在很大程度上未被探索.
研究的目的:
- 为了研究PD-1在新生儿带血衍生NK (CB-NK) 细胞上的表达和功能特征.
- 为了确定CB-NK细胞上的PD-1表达是否与孕妇细胞巨乳病毒 (CMV) 血清阳性或怀孕期间感染有关.
主要方法:
- 采用多参数流细胞计,分析新生儿CB-NK细胞的大队列上的PD-1表达.
- 根据CD56和其他相关标志物确定NK细胞子集.
- 进行了功能性测试,以评估PD-1触发对CB-NK细胞活性的影响.
主要成果:
- 在分析的CB-NK细胞中,PD-1被检测到超过一半,主要在CD56dim和CD56neg子集上.
- 与成人不同的是,CB-NK细胞上的PD-1与KIR和NKG2A共同表达.
- PD-1表达独立于母亲的CMV状态,并且没有证据表明胎儿的CMV感染或重新激活.
- 在CB-NK细胞上触发PD-1显示出功能抑制活性.
结论:
- 这项研究提供了第一份关于CB-NK细胞上PD-1表达的报告,并描述了其在围产期的特征.
- 功能性PD-1在CB-NK细胞上的存在,无论CMV感染如何,都表明它在早期发育期间在免疫调节中的潜在作用.
- 这些发现可能有助于选择特定的CB-NK细胞群体来开发新的免疫治疗策略.
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