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结构性调查关于多基菌体的客负荷如何影响与模拟肠道液体的相互作用

Sebastian Endres¹, Silvia Ehrmanntraut¹, Lukas Endres²

¹Institute of Organic Chemistry, University of Würzburg, Am Hubland, 97074 Würzburg, Germany.

ACS biomaterials science & engineering

|July 13, 2023

概括

了解药物负载如何影响肠道中的聚合物微粒是口服药物输送的关键. 这项研究揭示了药物胆汁相互作用和交换,对吸收至关重要,负载影响囊泡形成.

科学领域:

聚合物科学聚合物科学
生物物理化学生物物理化学
药物输送系统药物输送系统

背景情况:

药物加载显著影响聚合物微粒特性和生物性能.
口服药物输送需要了解肠道环境对这些纳米载体的影响.
在物质生物学界面的结构洞察力对于优化药物输送载体至关重要.

研究的目的:

在模拟的肠液中,以库尔库载入的多-2-氧沙) 菌粒进行结构性表征.
阐明黄素,胆盐,脂质和聚合物在材料生物学界面之间的相互作用.
为了研究黄素负载对菌组合和行为在养状态模拟肠液 (FeSSIF) 的影响.

主要方法:

核磁共振 (NMR) 谱学 (1H NMR,1H-1H-NOESY,1H DOSY). 通过核磁共振 (NMR) 进行测量.
低温传递电子显微镜 (cryo-TEM). 低温传递电子显微镜.
量子化学计算量子化学计算

主要成果:

鉴定了黄素和陶罗酸盐之间的关键相互作用,以及聚合物和脂质相互作用.
在聚合物微粒和胆结合物之间证明了黄素的交换,这对于药物吸收至关重要.
观察到,较高的黄素负载会导致更多的囊泡,因为黄酸对脂质纳米粒子形成的可用性较低.

结论:

黄素负载显著影响微粒结构和行为在模拟的肠道条件.
药物胆汁盐相互作用和交换对于口服吸收机制至关重要.
这些发现强调了需要进行多样化的药物和聚合物研究,以推进分子层面对口服药物输送的理解.

关键词:

这里是FeSSIFIF.核磁共振光谱法 (NMR) 是一种光谱法.生物相关的生物相关.结冷-TEM可以使用.含有药物的聚合物米塞尔.

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