在液体染色学中进行大规模保留测量的高吞吐量方法的表征
Trevor Kempen1, Tina Dahlseid1, Thomas Lauer1
1Gustavus Adolphus College, 800 W College Ave, St. Peter, MN 56082, USA.
Journal of chromatography. A
|July 13, 2023
概括
研究人员开发了一种高通量方法,使用短液态色谱列来生成大量保留数据的数据集. 这种方法可以提高数据的可访问性,从而开发出更好的染色体模型和工具.
科学领域:
- 分析化学 分析化学
- 染色学 科学 染色学 科学
- 分离科学 分离科学
背景情况:
- 由于实验保留数据有限,当代液体染色学在方法开发和理解染色学现象方面面临挑战.
- 获得保留数据的传统方法耗时且资源密集,阻碍了创建全面数据集.
研究的目的:
- 呈现大约13,000个液体染色学保留测量的数据集,使用高通量方法与短列获得.
- 为这种高通量测量技术引入一种改进的样本引入方法.
- 评估生成的保留数据的质量,可重复性和可重复性.
主要方法:
- 使用非常短的液态染色体列 (通常为5毫米),以增加测量吞吐量.
- 采用了一种新的样本引入方法,优化用于高通量保留测量.
- 包括对照化合物的重复测量,用于质量控制,异常值检测和测量一致性的评估.
主要成果:
- 创建了一个数据集,包括13000个保留测量35个小分子在9个静止相和各种移动相组合.
- 证明高可重复性,平均相对标准偏差 (RSD) 为0.4%,保留因子>1.
- 在六个月内表现出良好的可重现性,对非电离子化合物的选择性测量差异为+/- 1%.
结论:
- 选择性 (k_x/k_ref) 是一个比单独的保留因子更一致的长期保留的衡量标准.
- 高通量方法可以创建大型保留值数据库,这对于推进液态染色学模型和工具至关重要.
- 这种方法通过提供广泛,可靠的实验数据,有助于解决液体染色学中的关键挑战.
更多相关视频
07:34Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
12.8K
10:17High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
9.7K
相关概念视频
01:11High-Performance Liquid Chromatography: Introduction
2.1K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
2.1K
01:18Chromatographic Methods: Terminology
2.3K
Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
2.3K
00:57High-Performance Liquid Chromatography: Instrumentation
1.9K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
1.9K
01:05High-Performance Liquid Chromatography: Elution Process
540
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
540
01:15High-Performance Liquid Chromatography: Types of Detectors
624
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
624
01:13Principles Of Column Chromatography
6.9K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
6.9K