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单细胞空间转录组揭示了皮层中的细胞类型组织

Ao Chen ¹, Yidi Sun ², Ying Lei ³

¹BGI-Shenzhen, Shenzhen 518103, China; Department of Biology, University of Copenhagen, Copenhagen 2200, Denmark; BGI Research-Southwest, BGI, Chongqing 401329, China; JFL-BGI STOmics Center, Jinfeng Laboratory, Chongqing 401329, China.
²Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
³BGI-Shenzhen, Shenzhen 518103, China; BGI-Hangzhou, Hangzhou 310012, China.
⁴BGI-Shenzhen, Shenzhen 518103, China; BGI Research-Southwest, BGI, Chongqing 401329, China; JFL-BGI STOmics Center, Jinfeng Laboratory, Chongqing 401329, China.
⁵Lingang Laboratory, Shanghai 200031, China.
⁶CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
⁷BGI-Shenzhen, Shenzhen 518103, China.
⁸Tencent AI Lab, Shenzhen 518057, China.
⁹BGI-Shenzhen, Shenzhen 518103, China; School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
¹⁰BGI-Shenzhen, Shenzhen 518103, China; China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China.
¹¹BGI-Shenzhen, Shenzhen 518103, China; Guangdong Bigdata Engineering Technology Research Center for Life Sciences, Shenzhen 518083, China.
¹²China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China.
¹³Department of Biology, University of Copenhagen, Copenhagen 2200, Denmark.
¹⁴Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China; Shanghai Center for Brain Science and Brain-Inspired Technology, Shanghai 201602, China.
¹⁵Department of Protein Science, Science for Life Laboratory, KTH-Royal Institute of Technology, Stockholm 17121, Sweden; Department of Neuroscience, Karolinska Institute, Stockholm 17177, Sweden.
¹⁶Lingang Laboratory, Shanghai 200031, China; CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
¹⁷BGI-Shenzhen, Shenzhen 518103, China; BGI Research-Wuhan, BGI, Wuhan 430074, China.
¹⁸BGI-Shenzhen, Shenzhen 518103, China; Guangdong Provincial Key Laboratory of Genome Read and Write, Shenzhen 518120, China.
¹⁹Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China; Shanghai Center for Brain Science and Brain-Inspired Technology, Shanghai 201602, China; School of Future Technology, University of Chinese Academy of Sciences, Beijing 100049, China.

⁰BGI-Shenzhen, Shenzhen 518103, China; Department of Biology, University of Copenhagen, Copenhagen 2200, Denmark; BGI Research-Southwest, BGI, Chongqing 401329, China; JFL-BGI STOmics Center, Jinfeng Laboratory, Chongqing 401329, China.

Cell +

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2023年七月13日

在PubMed上查看摘要

概括

研究人员使用先进的测序测绘了大脑皮层中的264种细胞类型. 这个大脑图谱揭示了与大脑层次相关的细胞分布,并确定了灵长类动物特有的细胞类型,有助于未来的神经科学研究.

科学领域

神经科学
基因组学
细胞生物学

背景情况

了解大脑皮层的细胞组织是大脑功能的关键.
之前的研究缺乏细胞类型及其空间分布的全面地图.

研究的目的

创建一个关于皮细胞类型及其空间组织的详细地图.
研究细胞分布,皮质层次和灵长类细胞类型之间的关系.

主要方法

大规模的单核RNA测序
在143个皮区域进行空间转录组分析.
跨物种的转录组比较 (人类,,老鼠).

主要成果

264个转录组定义的皮质细胞类型的综合图谱.
在皮层中绘制细胞类型的空间分布.
在第4层中发现细胞分布,区域层次和灵长类特异性细胞类型之间的联系.

结论

提供灵长类大脑进化,发育和疾病的细胞和分子基础.
突出了皮层功能中的空间组织的重要性.
识别具有区域依赖基因表达的新型灵长类细胞.

关键词:

细胞类型组织层次结构的皮层一个细胞空间转录组