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高温通过Arabidopsis中的PIF4-miR166-HB15模块抑制血管发育

Hongbin Wei¹, Zhi Song¹, Yurong Xie²

¹Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, School of Life Sciences, Southwest University, Chongqing 400715, China.

Current biology : CB

|July 13, 2023

概括

高温会通过改变血管发育,导致植物的干部滞留. 植物染色体交互因子4 (PIF4) 通过调节miR166-HB15模块直接影响这个过程,影响茎强度和作物产量.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

植物血管系统对于结构和运输至关重要,适应环境变化.
高温对作物产量和质量产生负面影响,因为它会导致树干沉积,但潜在的分子机制尚不清楚.

研究的目的:

阐明高温调节血管发育和干部在阿拉比多普西斯的存放的分子机制.
确定关键的遗传因素,涉及到植物茎的高温应激反应.

主要方法:

研究的阿拉比多普西斯植物具有改变了植物染色体相互作用因子4 (PIF4) 的表达.
分析了MIR166和HB15的基因表达,以应对温度和PIF4水平.
使用体外和体内测试来确认PIF4与MIR166促进体结合.

主要成果:

过度表达PIF4导致血管束数量减少,并减少二次细胞壁的厚度,模仿高温效应.
高温和PIF4降低了MIR166的表达,增加了HB15的表达.
PIF4直接抑制MIR166基因表达,在血管发育中建立PIF4,miR166和HB15之间的联系.

结论:

涉及PIF4,miR166和HB15的直接分子通路调节血管发育和二次细胞壁形成.
这一途径对于调节高温压力下植物的茎存放易感性至关重要.
研究结果为改善作物对热应激的抵抗力提供了洞察力.

关键词:

HD-ZIPIII转录因子的转录因子在PIF4中,PIF4是PIF4.高温的高温的高温的高温 miR166 是一个166个字母.二次细胞壁中的二次细胞壁.树干的投放树干的投放血管发育血管发育

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