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纳米颗粒通过调节IPEC-J2细胞内质网膜应激来缓解脱氧尼瓦伦醇诱导的肠上皮屏障功能障碍

Xiaofan Song¹, Lei Qiao¹, Xina Dou¹

¹The Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.

|July 13, 2023

概括

纳米颗粒 (SeNPs) 通过PERK通路减轻氧化应激和内分泌网膜应激,保护免受脱氧 (DON) 诱导的肠壁损伤. 这为水产养殖中DON污染提供了潜在的解决方案.

科学领域:

毒理学毒理学毒理学
细胞生物学细胞生物学
纳米技术纳米技术

背景情况:

肠上皮屏障对健康至关重要,但容易受到除氧烯 (DON) 等真菌毒素的损害.
多是谷物中普遍存在的污染物,对人类和动物健康构成重大风险.
纳米颗粒 (SeNPs) 显示出有前途的保护剂对毒素诱导的细胞损伤.

研究的目的:

研究Lactobacillus casei合成的纳米颗粒 (SeNPs) 对DON诱导的肠上皮屏障功能障碍的保护作用.
阐明PERK介导的内质网膜应激 (ERS) 信号通路在SeNPs对DON的保护机制中的作用.

主要方法:

IPEC-J2细胞暴露于DON和/或SeNPs,治疗包括PERK通路激动剂和抑制剂.
评估的参数包括体电阻 (TEER),紧结蛋白表达,活性氧物种 (ROS) 含量,抗氧化能力和内细胞网膜 (ER) 形态.
通过测量p-PERK水平,评估了与ERS相关的PERK通路的激活.

主要成果:

暴露于DON显著增加了肠上皮的透性,减少了紧结蛋白,增加了ROS,降低了抗氧化能力,并诱导了ER结构损伤和PERK通路激活.
治疗SeNPs缓解了DON诱导的氧化应激,ER损伤和PERK通路激活,从而改善了肠道屏障功能.
PERK通路调节证实了它的参与;PERK抑制剂模仿了SeNPs对p-PERK表达的保护作用.

结论:

纳米颗粒 (SeNPs) 有效地减轻IPEC-J2细胞中脱氧尼瓦 (DON) 诱导的肠表皮屏障功能障碍.
SeNPs的保护机制与抑制内细胞网膜与压力相关的PERK信号通路密切相关.
在水产养殖行业中,SeNPs代表了管理DON污染的潜在策略.

关键词:

脱氧尼瓦伦醇 (deoxynivalenol) 是一种除氧化物.细胞内膜网膜应激压力是什么肠道屏障的功能肠道屏障的功能纳米粒子是一种纳米粒子.

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