在中国小 Eriocheir sinensis 中的一个 QM 蛋白质基因的分子特征和表达分析
Kexin Liu1, Zhe Qu1, Jingjie Hu2
1MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China; Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institute, Ocean University of China, Sanya 572024, China.
Fish & shellfish immunology
|July 13, 2023
概括
Eriocheir sinensis中的QM蛋白基因 (EsQM) 是高度保存的,并在血细胞中表达. EsQM对细菌感染和环境污染物做出反应,这表明它在关节动物防御机制中发挥了作用.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 环境科学 环境科学
背景情况:
- QM蛋白是一种已知的瘤抑制剂,在免疫反应中起作用.
- 研究Eriocheir sinensis中的QM蛋白基因功能对于了解关节动物免疫力至关重要.
研究的目的:
- 来自Eriocheir sinensis的QM蛋白基因 (EsQM) 的克隆和表征.
- 研究EsQM在对生物和环境刺激的反应中的表达模式和功能作用.
主要方法:
- 使用快速放大cDNA末端 (RACE) 克隆EsQM.
- 对EsQM蛋白序列和保存域的生物信息分析.
- 实时定量PCR (qPCR) 分析EsQM mRNA在不同组织中的表达和刺激后.
主要成果:
- EsQM cDNA 是 781 bp,编码 219 个氨基酸与保存域.
- 在所有组织中表达EsQMmRNA,在血细胞中表达最高.
- 血细胞中的EsQM表达被Aeromonas hydrophila和BDE-47显著上调,对细菌刺激的反应更快,更长.
结论:
- EsQM 是关节动物中高度保存的基因.
- EsQM在Eriocheir sinensis对抗细菌感染的免疫防御中发挥作用.
- EsQM参与应对环境污染,表明其在防御机制中的双重作用.
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