翻译后修改 β-氧丁化 调节 Ustilaginoidea virens 病毒性 毒性
Xiaoyang Chen1, Yuhang Duan2, Zhiyong Ren3
1Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Agricultural University, Hefei, China.
Molecular & cellular proteomics : MCP
|July 13, 2023
概括
lysine β-hydroxybutyrylation (Kbhb) 调节了 Ustilaginoidea virens.中的真菌毒性. 这种翻译后的修改影响了蛋白质活性和局部化,为真菌病原体控制提供了洞察力.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 植物病理学 植物病理学
背景情况:
- lysine β-hydroxybutyrylation (Kbhb) 是一种广泛的翻译后修饰,与基因转录和细胞增殖有关.
- 在植物病原菌中的Kbhb的功能,特别是在植物疾病中,在很大程度上是未被探索的.
研究的目的:
- 研究氨酸β-氧丁基化 (Kbhb) 在植物病原菌Ustilaginoidea virens.中的作用和调节机制.
- 确定参与真菌毒性和细胞过程中的Kbhb位点和蛋白质.
主要方法:
- 蛋白质组分析以确定Ustilaginoidea virens. virens中的Kbhb位点和蛋白质.
- 针对位点的突变发生,以研究Kbhb对特定蛋白质的功能影响 (UvSlt2,UvCdc10).
- 分子动态模拟以了解Kbhb.b.的结构效应.
- 酶检测用于识别去除Kbhb的酶 (希斯脱乙酶).
主要成果:
- 在U. virens. 852个蛋白质中确定了2204个Kbhb位点.
- 在UvSlt2和UvCdc10中Kbhb位点的突变导致激酶活性降低,蛋白质局部化发生变化,真菌毒性降低.
- 鉴定出依赖NAD+的基因素脱乙酶UvSirt2和UvSirt5是负责Kbhb去除的关键酶.
结论:
- 氨酸β-氧丁化在调节蛋白质功能,局部化和U. virens.中的酶活性方面发挥着关键作用.
- Kbhb是一种显著的翻译后修饰,影响了植物病原菌的毒性.
- 了解Kbhb通路为控制植物真菌病提供了新的目标.
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