提高口服胰岛素的输送路径,使用微波制造的基托基改性配方
Huda S AlSalem1, Nisreen M Abdulsalam2, Najla A Khateeb3
1Department of Chemistry, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
International journal of biological macromolecules
|July 13, 2023
概括
修改后的奇托桑纳米颗粒有效地通过口服输送胰岛素,显著降低了糖尿病大鼠的血糖水平. 这种新的方法证明了高胰岛素封装和生物可用性,可以改善糖尿病管理.
科学领域:
- 生物材料科学 生物材料科学
- 药物输送系统 药物输送系统
- 纳米技术纳米技术
背景情况:
- 由于胰岛素在胃肠道中降解,口服胰岛素的输送面临着挑战.
- 酸盐是药物输送的有希望的生物材料,但需要进行修改以提高其有效性.
- 开发有效的口服胰岛素配方对于改善糖尿病管理和患者遵从性至关重要.
研究的目的:
- 开发和表征新型酸-丁酸酸纳米颗粒 (Cs-DNS NPs) 用于增强口服胰岛素的输送.
- 评估胰岛素载Cs-DNSNP的体外和体内性能.
- 评估Cs-DNSNP在降低血清葡萄糖水平和改善胰岛素生物可用性的潜力.
主要方法:
- 奇托经过球磨,并用丁酸功能化,以创建Cs-DNSNP.
- 确定了Cs-DNSNP的粒子大小,泽塔潜力和胰岛素捕获效率 (EP%).
- 实验室胰岛素释放研究在各种pH值下进行.
- 在体内研究涉及给糖尿病大鼠注射胰岛素载Cs-DNSNP,以测量血清葡萄糖水平 (SGL),生物可用性 (BA%) 和药理可用性 (PA%).
主要成果:
- Cs-DNS NPs的水力动力学粒子大小为33.29 ± 5.08 nm,正泽塔电位为+35 ± 3.5 mV.
- 实现了99.3 ± 1.6%的高胰岛素捕获效率 (EP%).
- Cs-DNS NPs显示持续释放胰岛素,在酸性条件下保持92%,在更高的pH值下释放大量胰岛素.
- 在糖尿病大鼠中,注入胰岛素的Cs-DNSNP在3小时后将SGL降低了39.4 ± 0.6%,BA%为17.5 ± 0.31%,PA%为8.6 ± 0.8%.
结论:
- 修改的奇托桑纳米颗粒 (Cs-DNS NPs) 显示出有效的口服胰岛素输送的巨大潜力.
- 开发的Cs-DNSNP显示出出色的胰岛素封装和受控释放特性.
- 在体内研究证实了Cs-DNSNP在降低血糖水平和提高糖尿病大鼠胰岛素生物可用性的有效性,强调了它们的安全性和治疗前景.
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