转录合修复蛋白UVSSA的结构特征及其与TFIIH蛋白的相互作用
Hiral Mistry1, Shweta Kumari1, Vinod K Aswal2
1Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, India; Homi Bhabha National Institute, Anushaktinagar, Mumbai, India.
International journal of biological macromolecules
|July 13, 2023
概括
紫外线刺激的支架蛋白A (UVSSA) 在与转录因子IIH (TFIIH) 结合时经历了构造变化. 这种相互作用对于DNA修复至关重要,因为它促进了修复复合体与损伤部位的分离.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 结构生物学 结构生物学
背景情况:
- 紫外线刺激的支架蛋白A (UVSSA) 对于转录合核酸切割修复 (TC-NER) 是至关重要的.
- UVSSA是一种内在无序的蛋白质,调节TC-NER复合体的组合和解离.
- 对于UVSSA在招募转录因子IIH (TFIIH) 和随后的修复复杂动态中的作用的结构基础仍然不清楚.
研究的目的:
- 调查UVSSA与TFIIH (p62-PHD) 的p62子单元的Pleckstrin-Homology域相互作用的结构后果.
- 阐明在DNA修复过程中TFIIH结合时UVSSA的形状变化的作用.
主要方法:
- 生物物理技术,包括体外复合形成试验.
- 采用了小角度X射线散射 (SAXS) 和小角度中子散射 (SANS).
- 对对距离分布函数和旋转半径的分析.
主要成果:
- 在体外,UVSSA与p62-PHD形成一个稳定的复合体.
- 复杂的形成引发了UVSSA对距离分布的显著改变,并减少了它的旋转半径.
- 这些发现表明,在与p62-PHD.结合时,UVSSA发生了实质性的形状变化.
结论:
- 结合TFIIH会诱导UVSSA的显著形状变化.
- 假设这些UVSSA结构重组对于TC-NER复合物与DNA损伤部位的分离至关重要,从而促进修复.
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