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来自水培农场的Chromobacterium haemolyticum分离物的致病性和传播潜力

Ye Htut Zwe¹, Dan Li¹

¹Department of Food Science and Technology, National University of Singapore, Singapore 117543.

Journal of applied microbiology

|July 13, 2023

概括

染色菌血解菌株WI5在体外具有较高的致病性,但由于在生菜上的生存率较低和对干燥的敏感性,食物传播潜力有限. 诸如低酸和紫外线等水处理方法对这种病原体有效.

科学领域:

食品安全微生物学食品安全微生物学
细菌的病原性细菌的病原性
处理水的有效性水处理效率.

背景情况:

新兴的食源性病原体对公众健康构成重大风险.
了解Chromobacterium haemolyticum的毒性和传播途径对于预防疫情至关重要.
水培系统可能为绿叶蔬菜的病原体污染提供独特的途径.

研究的目的:

评估肠道中Chromobacterium haemolyticum WI5在体外的致病性.
为了确定C.haemolyticum WI5对常见的水消毒处理方法的耐药性.
评估C.haemolyticum WI5通过水培生长的绿叶蔬菜的食物传播潜力.

主要方法:

使用人类结肠细胞 (HCT116) 进行体外测定,以评估细胞病变效应和细胞附着.
测试耐化 (NaOCl) 和紫外线 (UV) 辐射的耐受性.
在不同的温度条件下评估C.haemolyticum WI5在生菜上的存活率.

主要成果:

在实验室中,C. haemolyticum WI5表现出显著的细胞病变效应和比Salmonella Typhimurium更高的细胞附着.
该细菌对NaOCl和紫外线治疗表现出耐药性,与大肠杆菌O157:H7和P. aeruginosa相比.
C. haemolyticum WI5 显示在生菜上的持久性较差,在4°C和25°C时数量迅速下降.

结论:

C. haemolyticum WI5 具有显著的毒性因素和对肠道的致病性.
氧化和紫外线处理对于在水中的C.haemolyticum消毒是有效的.
干燥耐受性有限和绿叶蔬菜的生存能力差,表明食物传播的可能性有限.

关键词:

这是一种Chromobacterium的细菌.肠道病变肠道病变肠道病变通过食物传播的食物.病原性病原性病原性传递性传递性的传递性通过水传播的水.

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