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Circ_TNFRSF21通过miR-214-3p/CHI3L1P促进cSCC转移和M2巨分极的发生

Jun Ma¹, Lei Huang¹, Yan-Bin Gao¹

¹Department of Burns, Nanfang Hospital, Southern Medical University, Guangdong Province, PR China.

Journal of dermatological science

|July 13, 2023

概括

循环RNA circ_TNFRSF21通过海绵化miR-214-3p促进皮肤状细胞癌 (cSCC),导致CHI3L1上调. 这个轴驱动cSCC的进展和转移,提供潜在的诊断和治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

皮肤状细胞癌 (cSCC) 是一种具有显著转移潜力的侵袭性癌症.
了解驱动cSCC的分子机制对于开发有效治疗方法至关重要.

研究的目的:

阐明circ_TNFRSF21/miR-214-3p/CHI3L1分子轴在cSCC的进展中的作用.
研究该轴对cSCC细胞行为和瘤发育的功能影响.

主要方法:

使用分子生物学技术对circ_TNFRSF21的表征.
通过qRT-PCR和IHC评估基因和M2巨细胞标记体的表达.
使用体外测试评估cSCC细胞的增殖,迁移和入侵.
在体内研究使用裸体老鼠异种移植模型来评估瘤生长和转移.
使用RNA免疫沉降和双光酶试验验证分子相互作用的验证.

主要成果:

在cSCC组织和细胞中,Circ_TNFRSF21和CHI3L1的上调,而miR-214-3p的下调.
Circ_TNFRSF21沉默或miR-214-3p过度表达抑制了cSCC细胞的增殖,迁移,入侵和M2巨细胞的极化.
Circ_TNFRSF21作为miR-214-3p的一个分子海绵,直接准CHI3L1.
在体内,circ_TNFRSF21 knockdown抑制了瘤生长和转移.

结论:

该circ_TNFRSF21/miR-214-3p/CHI3L1轴是cSCC进展的关键驱动因素,影响细胞增殖,迁移,入侵和M2巨细胞两极分化.
这一轴代表了新型诊断标记物和cSCC治疗策略的有希望的目标.

关键词:

CHI3L1 的意思是电路_TNFRSF2121 的情况皮肤状细胞癌瘤 M2巨细胞的两极分化在 miR-214-3p 中使用.

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