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Updated: Jul 23, 2025

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

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在KCNQ2和SCN1A中增加了通过单次切割全合一等离子体的原始编辑速率

N Dirkx1,2, Wout J Weuring3, E De Vriendt4

  • 1Applied & Translational Neurogenomics Group, VIB Center for Molecular Neurology, VIB, Antwerp, Belgium. nina.dirkx@uantwerpen.vib.be.

BMC biology
|July 13, 2023
PubMed
概括

这项研究通过使用新型等离子体来增强人类干细胞的原始编辑 (PE) 技术. 优化的单导向RNA (sgRNA) PE系统在患者衍生细胞中实现了高编辑效率和准确性,这对未来的治疗至关重要.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?发育性和性脑病变,发育性和性脑病变.在EF-1alfafa中使用EF-1alfa.这就是EIEEE.基因编辑 基因编辑人类诱导的多能干细胞干细胞.KCNQ2 的时间.单一性疾病是一种单一性疾病.主要编辑主要编辑这就是SCN1A.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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