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相关概念视频

Proteomics01:33

Proteomics

7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.5K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

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Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
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相关实验视频

Updated: Jul 23, 2025

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

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人类转录组和蛋白质组稳定的参考数据集.

Shaohua Lu1,2, Hong Lu3, Tingkai Zheng3

  • 1Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes and MOE Key Laboratory of Tumor Molecular Biology, Institute of Life and Health Engineering, Jinan University, Guangzhou, China. 356511612@qq.com.

Scientific data
|July 13, 2023
PubMed
概括

研究人员开发了一个稳定的人类肝细胞细胞系,MHCC97H,作为Omics技术的标准参考材料. 这一细胞系确保了可复制的转录组和蛋白质组分析,进步了精密医学.

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科学领域:

  • 生物医学科学 生物医学科学
  • 基因组学就是基因组学.
  • 蛋白质组学是指蛋白质组学.

背景情况:

  • 高通量omics技术对于生物医学进步至关重要.
  • 奥米克技术的可复制性较差,阻碍了它们的广泛应用.
  • 需要标准参考材料来验证omics工作流程.

研究的目的:

  • 为了识别和表征一个稳定的细胞系的omics标准参考材料.
  • 评估MHCC97H细胞系的长期转录组和蛋白组稳定性.
  • 为转录组和蛋白质组学研究提供可靠的参考数据集.

主要方法:

  • 培养和亚培养人类肝细胞细胞系MHCC97H9代.
  • 进行转录组分析以评估基因表达稳定性.
  • 使用数据依赖和数据独立的获取方法进行蛋白质组分析.

主要成果:

  • MHCC97H细胞系在亚培养世代中表现出高的转录基因稳定性 (r = 0.9830.997).
  • 蛋白质组分析表明,在不同的获取方法中,蛋白质组分析显示出优异的可重复性 (r = 0.966 0.994).
  • MHCC97H细胞系的稳定性在多个实验室和平台上得到了验证.

结论:

  • 细胞系MHCC97H作为一个强大的和可重复的标准参考材料用于omics研究.
  • 这种标准化材料和相关数据集将提高omics工作流程的准确性和可靠性.
  • 这些发现有助于推进奥米克技术在精密医学中的应用.