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人类转录组和蛋白质组稳定的参考数据集

Shaohua Lu^1,2, Hong Lu³, Tingkai Zheng³

¹Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes and MOE Key Laboratory of Tumor Molecular Biology, Institute of Life and Health Engineering, Jinan University, Guangzhou, China. 356511612@qq.com.

Scientific data

|July 13, 2023

概括

研究人员开发了一个稳定的人类肝细胞细胞系,MHCC97H,作为Omics技术的标准参考材料. 这一细胞系确保了可复制的转录组和蛋白质组分析,进步了精密医学.

科学领域:

生物医学科学生物医学科学
基因组学就是基因组学.
蛋白质组学是指蛋白质组学.

背景情况:

高通量omics技术对于生物医学进步至关重要.
奥米克技术的可复制性较差,阻碍了它们的广泛应用.
需要标准参考材料来验证omics工作流程.

研究的目的:

为了识别和表征一个稳定的细胞系的omics标准参考材料.
评估MHCC97H细胞系的长期转录组和蛋白组稳定性.
为转录组和蛋白质组学研究提供可靠的参考数据集.

主要方法:

培养和亚培养人类肝细胞细胞系MHCC97H9代.
进行转录组分析以评估基因表达稳定性.
使用数据依赖和数据独立的获取方法进行蛋白质组分析.

主要成果:

MHCC97H细胞系在亚培养世代中表现出高的转录基因稳定性 (r = 0.9830.997).
蛋白质组分析表明,在不同的获取方法中,蛋白质组分析显示出优异的可重复性 (r = 0.966 0.994).
MHCC97H细胞系的稳定性在多个实验室和平台上得到了验证.

结论:

细胞系MHCC97H作为一个强大的和可重复的标准参考材料用于omics研究.
这种标准化材料和相关数据集将提高omics工作流程的准确性和可靠性.
这些发现有助于推进奥米克技术在精密医学中的应用.

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