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前mRNA拼接顺序是预先确定的,并保持跨多intronic转录的拼接忠实性
Karine Choquet1, Autum R Baxter-Koenigs1, Sarah-Luisa Dülk2
1Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA.
Nature structural & molecular biology
|July 13, 2023
概括
在人体细胞中,多引子拼接遵循预先确定的顺序,在细胞类型中保存. 这种由结合体组件影响的拼接顺序,确保了长时间前传递 RNA 处理的准确性.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 在RNA生物学,RNA生物学.
背景情况:
- 内部切除可以通过许多组合途径发生,可能导致替代拼接.
- 在多引子转录中,引子去除的精确顺序尚未完全理解.
研究的目的:
- 为了研究人类细胞中多引入子前传递 RNA (mRNA 前) 主导的拼接顺序.
- 为了确定是否在不同细胞类型和分化阶段保存拼接顺序.
- 探索结合体组件和 cis 调节元件对结合顺序的影响.
主要方法:
- 分析人类细胞中的拼接模式.
- 在运动神经元分化过程中研究剪接顺序的保存.
- 扰乱结合体U2小核RNA (snRNA) 功能.
- 研究影响内切除的 cis 调节机制.
主要成果:
- 大多数人类基因都表现出一个或几个占主导地位的多引子拼接顺序.
- 这些拼接顺序在各种细胞类型中和运动神经元分化过程中得到保护.
- 与替代拼接的外子相邻的内子通常是最后被切除的.
- 在U2 snRNA中发生的变化会影响拼接顺序,并可能导致内质保留.
- 特定的内部去除可以通过不同的cis调节机制诱导其他内部的延迟切除.
结论:
- 人类细胞中的多引子拼接顺序在很大程度上是预先确定的.
- 拼接顺序受到拼接体组件的影响,例如U2 snRNA.
- 预先确定的拼接顺序有助于长期前mRNAs的拼接忠实性.
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