发现和机制指导的工程BHET化剂,以改善PET回收和再循环
Anni Li1, Yijie Sheng1, Haiyang Cui2,3
1School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, 210009, People's Republic of China.
Nature communications
|July 14, 2023
概括
研究人员设计了称为BHETases的酶,以提高塑料回收效率. 这些增强的酶,称为ΔBHETases,使塑料在回收和再循环应用中能够更有效地分解.
科学领域:
- 生物技术是生物技术.
- 生物催化剂是一种生物催化剂.
- 聚合物科学 聚合物科学
背景情况:
- 酶性塑料降解面临着不完全分解和低效率的挑战.
- 聚乙烯二甲 (PET) 的回收和再循环需要更高效的酶溶液.
研究的目的:
- 为了识别和工程酶 (BHETases) 提高PET降解.
- 提高塑料回收和再循环的酶的效率和稳定性.
主要方法:
- 酶开采用于识别新型BHETase (ChryBHETase和BsEst).
- 机制引导的蛋白质工程,以创建热稳定的ΔBHETases,以提高催化效率 (kcat/KM).
- 开发一种两种酶系统和一种双重化学酶的方法,用于塑料的价值化.
主要成果:
- 确定了两种新型BHETase,并将它们改造为强大的,恒温稳定的 ΔBHETase,其催化效率高达 3.5 倍.
- 使用 ΔBHETase 的两种酶系统,与最先进的 PET 酸盐相比,可以达到高达 7.0 倍的 TPA 生产.
- 通过使用化学酶法组合的方法,成功地将21种消费后塑料转化为原始PET和p-phthaloyl化物.
结论:
- 工程BHETases为有效的PET回收和再循环提供了一个有希望的解决方案.
- 开发的酶和化学酶策略使得PET的闭环循环回收和开放循环循环回收成为可能.
- 这项工作推进了通过改进塑料废物回收利用来解决塑料危机的酶方法.
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