相关实验视频

Updated: Jul 31, 2026

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

一个新的微RNA小组的分析验证,用于认知障碍的风险分层

Arzu Kunwar¹, Kenny Kwabena Ablordeppey¹, Alidad Mireskandari¹

¹DiamiR Biosciences Laboratory, 2 Church Street South, Suite B05, New Haven, CT 06519, USA.

Diagnostics (Basel, Switzerland)

|July 14, 2023

概括

这项研究验证了CogniMIR® microRNA (miRNA) 面板用于识别认知障碍生物标志物. 这两种定量PCR技术都可靠地检测血中的目标miRNA,基于LNA的测试更适合临床实验室.

科学领域:

生物化学生物化学
分子生物学分子生物学
神经科学是一个神经科学.

背景情况:

微RNAs (miRNAs) 是认知障碍的新兴生物标志物.
基于等离子体的miRNA分析提供了一种非侵入性诊断方法.
科尼米尔 (CogniMIR) 面板的目标是大脑丰富和与炎症相关的miRNAs.

研究的目的:

为了分析验证新的CogniMIR®miRNA面板.
为了比较两个定量PCR (qPCR) 技术用于miRNA表达分析.
评估在血中检测miRNA的可靠性和可重复性.

主要方法:

使用了来自认知正常和认知受损个体的血样本.
使用MagMAX mirVana Kit进行了总RNA的分离.
用TaqMan (基于茎环) 和基于LNA的测定对24个目标miRNA进行RT-qPCR.

主要成果:

两种qPCR技术都可靠地检测到所有24个CogniMIR®面板miRNAs,减少到20个副本.
在运营商和日间观察到高重复性 (R2:0.94-0.99) 和可重复性 (R2:0.96-0.97).
基于LNA的qPCR在临床实验室环境中表现出更大的操作友好性.

结论:

科尼米尔 (CogniMIR) 面板经过分析验证,可用于血miRNA分析.
基于TaqMan和LNA的qPCR技术都适合用于miRNA表达分析.
基于LNA的qPCR因其在认证的临床实验室中的效率而被推.

关键词:

阿尔茨海默氏症 (AD) 是一种疾病.基于LNA的qPCRR可以使用.基于 TaqMan 的 qPCR 基因.分析验证的分析验证基于血液的分子诊断.认知障碍是一种认知障碍.循环miRNA的表达概况.微型RNA生物标记物轻度认知障碍 (MCI) 是一种轻度认知障碍.神经退行性疾病的神经退行性疾病

更多相关视频

A Machine Learning Approach to Design an Efficient Selective Screening of Mild Cognitive Impairment

A Machine Learning Approach to Design an Efficient Selective Screening of Mild Cognitive Impairment

Published on: January 11, 2020

Author Spotlight: Exploring Sex-Specific Glial Signatures and Therapeutic Leads for Alzheimer's Disease

Author Spotlight: Exploring Sex-Specific Glial Signatures and Therapeutic Leads for Alzheimer's Disease

Published on: May 20, 2024

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...