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在具有KRT71突变的CRISPR工程突变小鼠模型中发育毛发和无毛发

Tao Zhang¹, Hongwu Yao¹, Hejun Wang¹

¹Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.

|July 14, 2023

概括

研究人员使用CRISPR/Cas9基因编辑创建了一种用于羊毛 (WH) 和低三症简单 (HS) 的新型小鼠模型. 这些Krt71-knockout小鼠表现出卷发和脱发,有助于发型疾病研究.

科学领域:

遗传学遗传学是一个
皮肤病学皮肤病学
分子生物学分子生物学

背景情况:

简单的低发症 (HS) 和羊毛发 (WH) 是罕见的遗传性脱发疾病.
质蛋白KRT71基因的突变与HS和WH有关.

研究的目的:

使用CRISPR/Cas9基因编辑生成HS和WH的小鼠模型.
研究KRT71基因在头发发育和疾病中的作用.

主要方法:

克里斯普尔/卡斯9系统被用来准小鼠囊中Krt71基因的第6个外基因.
产生了Krt71-Knockout (KO) 的小鼠,并对表型变化进行分析.

主要成果:

Krt71-KO小鼠在3-5周后表现出卷发和完全脱发,模仿HS和WH表型.
在KO小鼠中,Krt71蛋白表达被删除.
尽管毛发脱落,但这些小鼠没有表现出免疫缺陷,这与裸体小鼠有所区别.

结论:

一个新的CRISPR/Cas9生成的小鼠模型准确地模仿了羊毛和简单的低三症.
这种Krt71-KO小鼠模型是研究毛发疾病机制的宝贵工具.

关键词:

这就是CRISPR/Cas9的作用.在 KRT71 里面.没有头发,没有头发.鼠标模型鼠标模型鼠标模型羊毛的头发是羊毛的

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相关概念视频

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.